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Clonal analysis in mice underlines the importance of rhombomeric boundaries in cell movement restriction during hindbrain segmentation

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dc.contributor.author Jiménez Guri, Eva
dc.contributor.author Udina, Frederic
dc.contributor.author Colas, Jean-François
dc.contributor.author Sharpe, James
dc.contributor.author Padrón Barthe, Laura
dc.contributor.author Torres, Miguel S.
dc.contributor.author Pujades Corbi, Cristina
dc.date.accessioned 2015-04-29T07:24:11Z
dc.date.available 2015-04-29T07:24:11Z
dc.date.issued 2010
dc.identifier.citation Jimenez-Guri E, Udina F, Colas J, Sharpe J, Padron-Barthe L, Torres M, Pujades C. Clonal analysis in mice underlines the importance of rhombomeric boundaries in cell movement restriction during hindbrain segmentation. PLoS ONE. 2010;5(4):e10112. DOI: 10.1371/journal.pone.0010112
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10230/23487
dc.description.abstract Background: Boundaries that prevent cell movement allow groups of cells to maintain their identity and follow independent developmental trajectories without the need for ongoing instructive signals from surrounding tissues. This is the case of vertebrate rhombomeric boundaries. Analysis in the developing chick hindbrain provided the first evidence that rhombomeres are units of cell lineage. The appearance of morphologically visible rhombomeres requires the segment restricted expression of a series of transcription factors, which position the boundaries and prefigure where morphological boundaries will be established. When the boundaries are established, when the cells are committed to a particular rhombomere and how they are organized within the hindbrain are important questions to our understanding of developmental regionalization. Methodology/Principal Findings: Sophisticated experimental tools with high-resolution analysis have allowed us to explore cell lineage restriction within the hindbrain in mouse embryos. This novel strategy is based on knock-in alleles of ubiquitous expression and allows unrestricted clonal analysis of cell lineage from the two-cell stage to the adult mouse. Combining this analysis with statistical and mathematical tools we show that there is lineage compartmentalization along the anteroposterior axis from very early stages of mouse embryonic development. Conclusions: Our results show that the compartment border coincides with the morphological boundary in the mouse hindbrain. The restriction of the cells to cross rhombomeric boundaries seen in chick is also observed in mouse. We show that the rhombomeric boundaries themselves are involved in cell movement restriction, although an underlying pre-pattern during early embryonic development might influence the way that cell populations organize
dc.description.sponsorship EJG was a recipient of a postdoctoral fellowship Beatriu de Pinos (Generalitat de Catalunya, Spain). The work was funded by MTM2009-09063 to FU, BFU2007-62744/BMC (MICINN, Spain) to JS, MADRICEL S-SAL-0190-2006 (Madrid Regional Government) to MT and BFU2009-07010 (MICINN, Spain) to CP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS ONE. 2010;5(4):e10112
dc.rights © 2010 Jimenez-Guri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
dc.subject.other Genètica animal
dc.title Clonal analysis in mice underlines the importance of rhombomeric boundaries in cell movement restriction during hindbrain segmentation
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0010112
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/MTM2009-09063
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2007-62744
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/SAL2006-0190
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-07010
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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