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Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe

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dc.contributor.author Sansó Martínez, Miriam, 1979-
dc.contributor.author Vargas Pérez, Itzel
dc.contributor.author Quintales, Luis
dc.contributor.author Antequera, Francisco
dc.contributor.author Ayté del Olmo, José
dc.contributor.author Hidalgo Hernando, Elena
dc.date.accessioned 2015-04-07T09:36:00Z
dc.date.available 2015-04-07T09:36:00Z
dc.date.issued 2011
dc.identifier.citation Samsó M, Vargas-Perez I, Quintales L, Antequera F, Ayte J, Hidalgo E. Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Research. 2011;39(15):6369-79. DOI: 10.1093/nar/gkr255
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10230/23338
dc.description.abstract In the fission yeast, the MAP kinase Sty1 and the transcription factor Atf1 regulate up to 400 genes in response to environmental signals, and both proteins have been shown to bind to their promoters in a stress-dependent manner. In a genetic search, we have isolated the histone H3 acetyltransferase Gcn5, a component of the SAGA complex, as being essential for oxidative stress survival and activation of those genes. Upon stress, Gcn5 is recruited to promoters and coding sequences of stress genes in a Sty1- and Atf1-dependent manner, causing both an enhanced acetylation of histone H3 and nucleosome eviction. Unexpectedly, recruitment of RNA polymerase II (Pol II) is not impaired in Δgcn5 cells. We show here that stress genes display a 400-bp long nucleosome depleted region upstream of the transcription start site even prior to activation. Stress treatment does not alter promoter nucleosome architecture, but induces eviction of the downstream nucleosomes at stress genes, which is not observed in Δgcn5 cells. We conclude that, while Pol II is recruited to nucleosome-free stress promoters in a transcription factor dependent manner, Gcn5 mediates eviction of nucleosomes positioned downstream of promoters, allowing efficient Pol II progression along the genes.
dc.description.sponsorship Funding for open access charge: Spanish Ministry of Science and Innovation (BFU2009-06933), PLAN E and FEDER, by the Spanish program Consolider-Ingenio 2010 Grant CSD 2007-0020, and by SGR2009-196 from Generalitat de Catalunya (Spain) (to E.H.). E.H. and J.A. are recipients of an ICREA Academia Award (Generalitat de Catalunya)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartof Nucleic Acids Research. 2011;39(15):6369-79
dc.rights © Samsó M, Vargas-Perez I, Quintales L, Antequera F, Ayte J, Hidalgo E [2011]. Published by Oxford University Press. This is an Open Access article distributed under the terms of a Creative Commons Attribution License
dc.rights.uri http://creativecommons.org/licenses/by-nc/2.5
dc.subject.other Schizosaccharomyces pombe -- Metabolisme
dc.subject.other Proteïnes quinases activades per mitògens
dc.subject.other Factors de transcripció
dc.title Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1093/nar/gkr255
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-06933
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/CSD2007-0020
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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