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A quantitative atlas of Even-skipped and Hunchback expression in Clogmia albipunctata (Diptera: Psychodidae) blastoderm embryos

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dc.contributor.author Janssens, Hilde
dc.contributor.author Siggens, Ken
dc.contributor.author Cicin Sain, Damjan
dc.contributor.author Jiménez Guri, Eva
dc.contributor.author Musy, Marco
dc.contributor.author Akam, Michael
dc.contributor.author Jaeger, Johannes, 1973-
dc.date.accessioned 2015-03-18T09:12:18Z
dc.date.available 2015-03-18T09:12:18Z
dc.date.issued 2014
dc.identifier.citation Janssens H, Siggens K, Cicin-Sain D, Jiménez-Guri E, Musy M, Akam M, Jaeger J. A quantitative atlas of Even-skipped and Hunchback expression in Clogmia albipunctata (Diptera: Psychodidae) blastoderm embryos. EvoDevo. 2014; 5: 1. DOI 10.1186/2041-9139-5-1
dc.identifier.issn 2041-9139
dc.identifier.uri http://hdl.handle.net/10230/23219
dc.description.abstract Background: Comparative studies of developmental processes are one of the main approaches to evolutionary developmental biology (evo-devo). Over recent years, there has been a shift of focus from the comparative study of particular regulatory genes to the level of whole gene networks. Reverse-engineering methods can be used to computationally reconstitute and analyze the function and dynamics of such networks. These methods require quantitative spatio-temporal expression data for model fitting. Obtaining such data in non-model organisms remains a major technical challenge, impeding the wider application of data-driven mathematical modeling to evo-devo. Results: We have raised antibodies against four segmentation gene products in the moth midge Clogmia albipunctata, a non-drosophilid dipteran species. We have used these antibodies to create a quantitative atlas of protein expression patterns for the gap gene hunchback (hb), and the pair-rule gene even-skipped (eve). Our data reveal differences in the dynamics of Hb boundary positioning and Eve stripe formation between C. albipunctata and Drosophila melanogaster. Despite these differences, the overall relative spatial arrangement of Hb and Eve domains is remarkably conserved between these two distantly related dipteran species. Conclusions: We provide a proof of principle that it is possible to acquire quantitative gene expression data at high accuracy and spatio-temporal resolution in non-model organisms. Our quantitative data extend earlier qualitative studies of segmentation gene expression in C. albipunctata, and provide a starting point for comparative reverse-engineering studies of the evolutionary and developmental dynamics of the segmentation gene system.
dc.description.sponsorship Work in Cambridge and Barcelona was funded by the UK Biotechnology and Biological Sciences Research Council (grant number BB/D00513), by the MEC-EMBL agreement for the EMBL/CRG Research Unit in Systems Biology, SGR Grant 406 from the Catalan funding agency AGAUR, and by grants BFU2009-10184 and BFU2012-33775 from the Spanish Ministerio de Economia y Competitividad (MINECO; formerly MICINN)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartof EvoDevo. 2014; 5: 1
dc.rights © 2013 Janssens et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.uri http://creativecommons.org/licenses/by/2.0
dc.subject.other Bioinformàtica
dc.subject.other Genètica evolutiva
dc.title A quantitative atlas of Even-skipped and Hunchback expression in Clogmia albipunctata (Diptera: Psychodidae) blastoderm embryos
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1186/2041-9139-5-1
dc.subject.keyword Clogmia albipunctata
dc.subject.keyword Non-drosophilid diptera
dc.subject.keyword Non-model organism
dc.subject.keyword Pattern formation
dc.subject.keyword Comparative network analysis
dc.subject.keyword Segmentation gene network
dc.subject.keyword Hunchback
dc.subject.keyword Even-skipped
dc.subject.keyword Image bioinformatics
dc.subject.keyword Quantitative expression data
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-10184
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-33775
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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