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Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface

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dc.contributor.author Wakana, Yuichi
dc.contributor.author Villeneuve, Julien
dc.contributor.author Galen, Josse van
dc.contributor.author Cruz-Garcia, David
dc.contributor.author Tagaya, Mitsuo
dc.contributor.author Malhotra, Vivek
dc.date.accessioned 2014-05-08T09:17:04Z
dc.date.available 2014-05-08T09:17:04Z
dc.date.issued 2013
dc.identifier.citation Wakana Y, Villeneuve J, van Galen J, Cruz-Garcia D, Tagaya M, Malhotra V. Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface. J Cell Biol. 2013;202(2):241-50. DOI: 10.1083/jcb.201303163
dc.identifier.issn 0021-9525
dc.identifier.uri http://hdl.handle.net/10230/22477
dc.description.abstract Here we report that the kinesin-5 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for protein transport from the Golgi complex to the cell surface in Drosophila S2 cells. Disrupting the function of its mammalian orthologue, Eg5, in HeLa cells inhibited secretion of a protein called pancreatic adenocarcinoma up-regulated factor (PAUF) but, surprisingly, not the trafficking of vesicular stomatitis virus G protein (VSV-G) to the cell surface. We have previously reported that PAUF is transported from the trans-Golgi network (TGN) to the cell surface in specific carriers called CARTS that exclude VSV-G. Inhibition of Eg5 function did not affect the biogenesis of CARTS; however, their migration was delayed and they accumulated near the Golgi complex. Altogether, our findings reveal a surprising new role of Eg5 in nonmitotic cells in the facilitation of the transport of specific carriers, CARTS, from the TGN to the cell surface.
dc.description.sponsorship J. Villeneuve is funded by a long-term EMBO postdoctoral fellowship. V. Malhotra is an Institució Catalana de Recerca i Estudis Avançats (ICREA) professor at the Centre for Genomic Regulation, and the work in his laboratory is funded by grants from Plan Nacional (BFU2008-00414), Consolider (CSD2009-00016), and European Research Council (268692)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Rockefeller University Press
dc.relation.ispartof Journal of cell biology. 2013;202(2):241-50
dc.rights © Yuichi Wakana et al., 2013. This article is distributed under a/nCreative Commons License Attribution–Noncommercial–Share Alike 3.0 Unported.
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject.other Proteïnes
dc.subject.other Aparell de Golgi
dc.title Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1083/jcb.201303163
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/268692
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2008-00414
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CSD2009-00016
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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