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Fusion of the human gene for the polyubiquitination co-effector UEV-1 with Kua, a newly identified gene

Mostra el registre parcial de l'element Thomson, Timothy M. Lozano, Juan José Loukili, Noureddine Carrió, Roberto Serras Rigalt, Florenci Cormand, Bru Valeri, Marta Díaz, Víctor M. Abril Ferrando, Josep Francesc Burset Albareda, Moisès Merino, Jesús Macaya, Alfons Corominas Guiu, Montserrat Guigó Serra, Roderic 2012-05-21T09:44:54Z 2012-05-21T09:44:54Z 2000
dc.identifier.citation Thomson TM, Lozano JJ, Loukili N, Carrió R, Serras F, Cormand B, Valeri M, Díaz VM, Abril JF, Burset M, Merino J, Macaya A, Corominas M, Guigó R. Fusion of the human gene for the polyubiquitination co-effector UEV-1 with Kua, a newly identified gene. Genome Res. 2000; 10(11): 1743-56. DOI:/n10.1101/gr.140500
dc.identifier.issn 1088-9051
dc.description.abstract UEV proteins are enzymatically inactive variants of the E2 ubiquitin-conjugating enzymes that regulate noncanonical elongation of ubiquitin chains. In Saccharomyces cerevisiae, UEV is part of the RAD6-mediated error-free DNA repair pathway. In mammalian cells, UEV proteins can modulate c-FOS transcription and the G2-M transition of the cell cycle. Here we show that the UEV genes from phylogenetically distant organisms present a remarkable conservation in their exon–intron structure. We also show that the human UEV1 gene is fused with the previously unknown gene Kua. In Caenorhabditis elegans and Drosophila melanogaster, Kua and UEV are in separated loci, and are expressed as independent transcripts and proteins. In humans, Kua and UEV1 are adjacent genes, expressed either as separate transcripts encoding independent Kua and UEV1 proteins, or as a hybrid Kua–UEV transcript, encoding a two-domain protein. Kua proteins represent a novel class of conserved proteins with juxtamembrane histidine-rich motifs. Experiments with epitope-tagged proteins show that UEV1A is a nuclear protein, whereas both Kua and Kua–UEV localize to cytoplasmic structures, indicating that the Kua domain determines the cytoplasmic localization of Kua–UEV. Therefore, the addition of a Kua domain to UEV in the fused Kua–UEV protein confers new biological properties to this regulator of variant polyubiquitination./n/n[Kua cDNAs isolated by RT-PCR and described in this paper have been deposited in the GenBank data library under accession nos. AF1155120 (H. sapiens) and AF152361 (D. melanogaster). Genomic clones containing UEV genes: S. cerevisiae, YGL087c (accession no. Z72609); S. pombe, c338 (accession no. AL023781); P. falciparum, MAL3P2 (accession no. AL034558); A. thaliana, F26F24 (accession no. AC005292); C. elegans, F39B2 (accession no. Z92834); D. melanogaster, AC014908; and H. sapiens, 1185N5 (accession no. AL034423). Accession numbers for Kua cDNAs in GenBank dbEST: M. musculus, AA7853; T. cruzi, AI612534. Other Kua-containing sequences: A. thaliana genomic clones F10M23 (accession no. AL035440), F19K23 (accession no. AC000375), and T20K9 (accession no. AC004786).
dc.language.iso eng
dc.publisher Cold Spring Harbor Laboratory Press-CSHL Press
dc.relation.ispartof Genome Res. 2000; 10(11): 1743-56
dc.rights © 2000 Genome Research by Cold Spring Harbor Laboratory Press. Published version available at Aquest document està subjecte a Llicència Creative Commons (Attribution-NonCommercial 3.0 Unported License)
dc.subject.other Factors de transcripció
dc.subject.other Genètica humana
dc.title Fusion of the human gene for the polyubiquitination co-effector UEV-1 with Kua, a newly identified gene
dc.type info:eu-repo/semantics/article
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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