Articles (Center for Genomic Regulation (CRG))http://hdl.handle.net/10230/222272024-03-19T02:12:43Z2024-03-19T02:12:43ZDistinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeastMasgrau Fortuny, Aina, 1986-Battola, Andrea, 1987-Sanmartín Olmo, TrinidadPryszcz, Leszek Piotr, 1985-Gabaldón Estevan, Juan Antonio, 1973-Mendoza, Manuel (Mendoza Palomares)http://hdl.handle.net/10230/592552024-02-27T02:30:50Z2017-01-01T00:00:00ZDistinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeast
Masgrau Fortuny, Aina, 1986-; Battola, Andrea, 1987-; Sanmartín Olmo, Trinidad; Pryszcz, Leszek Piotr, 1985-; Gabaldón Estevan, Juan Antonio, 1973-; Mendoza, Manuel (Mendoza Palomares)
Boi1 and Boi2 (Boi1/2) are budding yeast plasma membrane proteins that function in polarized growth, and in cytokinesis inhibition in response to chromosome bridges via the NoCut abscission checkpoint. How Boi1/2 act in these two distinct processes is not understood. We demonstrate that Boi1/2 are required for a late step in the fusion of secretory vesicles with the plasma membrane of the growing bud. Cells lacking Boi1/2 accumulate secretory vesicles and are defective in bud growth. In contrast, Boi2 is specifically required for abscission inhibition in cells with chromatin bridges. The SH3 domain of Boi2, which is dispensable for bud growth and targets Boi2 to the site of abscission, is necessary and sufficient for abscission inhibition. Gain of function of the exocyst, a conserved protein complex involved in tethering of exocytic vesicles to the plasma membrane, rescued secretion and bud growth defects in boi mutant cells, and abrogated NoCut checkpoint function. Thus Boi2 functions redundantly with Boi1 to promote the fusion of secretory vesicles with the plasma membrane at sites of polarized growth, and acts as an abscission inhibitor during cytokinesis in response to chromatin bridges.
2017-01-01T00:00:00ZThe genome of the generalist plant pathogen fusarium avenaceum is enriched with genes involved in redox, signaling and secondary metabolismLysøe, ErikHarris, Linda J.Walkowiak, SeanSubramaniam, RajagopalDivon, Hege H.Riiser, Even S.Llorens, Carlos, 1968-Gabaldón Estevan, Juan Antonio, 1973-Kistler, H. CorbyJonkers, WilfriedKolseth, Anna-KarinNielsen, Kristian F.Thrane, UlfFrandsen, Rasmus J. N.http://hdl.handle.net/10230/591692024-02-21T02:30:57Z2014-01-01T00:00:00ZThe genome of the generalist plant pathogen fusarium avenaceum is enriched with genes involved in redox, signaling and secondary metabolism
Lysøe, Erik; Harris, Linda J.; Walkowiak, Sean; Subramaniam, Rajagopal; Divon, Hege H.; Riiser, Even S.; Llorens, Carlos, 1968-; Gabaldón Estevan, Juan Antonio, 1973-; Kistler, H. Corby; Jonkers, Wilfried; Kolseth, Anna-Karin; Nielsen, Kristian F.; Thrane, Ulf; Frandsen, Rasmus J. N.
Fusarium avenaceum is a fungus commonly isolated from soil and associated with a wide range of host plants. We present here three genome sequences of F. avenaceum, one isolated from barley in Finland and two from spring and winter wheat in Canada. The sizes of the three genomes range from 41.6–43.1 MB, with 13217–13445 predicted protein-coding genes. Whole-genome analysis showed that the three genomes are highly syntenic, and share>95% gene orthologs. Comparative analysis to other sequenced Fusaria shows that F. avenaceum has a very large potential for producing secondary metabolites, with between 75 and 80 key enzymes belonging to the polyketide, non-ribosomal peptide, terpene, alkaloid and indole-diterpene synthase classes. In addition to known metabolites from F. avenaceum, fuscofusarin and JM-47 were detected for the first time in this species. Many protein families are expanded in F. avenaceum, such as transcription factors, and proteins involved in redox reactions and signal transduction, suggesting evolutionary adaptation to a diverse and cosmopolitan ecology. We found that 20% of all predicted proteins were considered to be secreted, supporting a life in the extracellular space during interaction with plant hosts.
2014-01-01T00:00:00ZState of art of cancer pharmacogenomics in Latin American populationsLópez-Cortés, AndrésGuerrero, Santiago, 1957-Redal, María AnaAlvarado, Angel TitoQuiñones, Luishttp://hdl.handle.net/10230/591682024-02-21T02:30:53Z2017-01-01T00:00:00ZState of art of cancer pharmacogenomics in Latin American populations
López-Cortés, Andrés; Guerrero, Santiago, 1957-; Redal, María Ana; Alvarado, Angel Tito; Quiñones, Luis
Over the past decades, several studies have shown that tumor-related somatic and germline alterations predicts tumor prognosis, drug response and toxicity. Latin American populations present a vast geno-phenotypic diversity due to the great interethnic and interracial mixing. This genetic flow leads to the appearance of complex characteristics that allow individuals to adapt to endemic environments, such as high altitude or extreme tropical weather. These genetic changes, most of them subtle and unexplored, could establish a mutational profile to develop new pharmacogenomic therapies specific for Latin American populations. In this review, we present the current status of research on somatic and germline alterations in Latin America compared to those found in Caucasian and Asian populations.
2017-01-01T00:00:00ZDistinct structural transitions of chromatin topological domains correlate with coordinated hormone-induced gene regulationLe Dily, FrançoisBaù, DavidePohl, Andy, 1979-Vicent, Guillermo PabloSerra, FrançoisSoronellas, DanielCastellano, GiancarloWright, Roni H.G.Ballare, Cecilia JuliaFilion, GuillaumeMarti-Renom, Marc A.Beato, Miguelhttp://hdl.handle.net/10230/591572024-02-21T02:31:04Z2014-01-01T00:00:00ZDistinct structural transitions of chromatin topological domains correlate with coordinated hormone-induced gene regulation
Le Dily, François; Baù, Davide; Pohl, Andy, 1979-; Vicent, Guillermo Pablo; Serra, François; Soronellas, Daniel; Castellano, Giancarlo; Wright, Roni H.G.; Ballare, Cecilia Julia; Filion, Guillaume; Marti-Renom, Marc A.; Beato, Miguel
The human genome is segmented into topologically associating domains (TADs), but the role of this conserved organization during transient changes in gene expression is not known. Here we describe the distribution of progestin-induced chromatin modifications and changes in transcriptional activity over TADs in T47D breast cancer cells. Using ChIP-seq (chromatin immunoprecipitation combined with high-throughput sequencing), Hi-C (chromosome capture followed by high-throughput sequencing), and three-dimensional (3D) modeling techniques, we found that the borders of the ∼ 2000 TADs in these cells are largely maintained after hormone treatment and that up to 20% of the TADs could be considered as discrete regulatory units where the majority of the genes are either transcriptionally activated or repressed in a coordinated fashion. The epigenetic signatures of the TADs are homogeneously modified by hormones in correlation with the transcriptional changes. Hormone-induced changes in gene activity and chromatin remodeling are accompanied by differential structural changes for activated and repressed TADs, as reflected by specific and opposite changes in the strength of intra-TAD interactions within responsive TADs. Indeed, 3D modeling of the Hi-C data suggested that the structure of TADs was modified upon treatment. The differential responses of TADs to progestins and estrogens suggest that TADs could function as "regulons" to enable spatially proximal genes to be coordinately transcribed in response to hormones.
Includes supplementary materials for the online appendix.
2014-01-01T00:00:00Z