Browsing by Author "Vicent, Guillermo Pablo"

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  • A 3D culture system to study gene regulation in breast cancer cells
    Ramírez Cuéllar, Angélica Julieta, 1990- (2022-05-12)
    In this Thesis we report the successful growth of hormone responsive T47D cells as 3D spheroids in Matrigel mimicking the ECM. A detailed characterization by using a genome-wide approach of the main physical and biochemical ...
  • C/EBPalpha activates pre-existing and de novo macrophage enhancers during induced pre-B cell transdifferentiation and myelopoiesis
    van Oevelen, Chris; Collombet, Samuel; Vicent, Guillermo Pablo; Hoogenkamp, Maarten; Lepoivre, Cyrille; Badeaux, Aimee; Bussmann, Lars; Sardina, Jose Luis; Thieffry, Denis; Beato, Miguel; Shi, Yang; Bonifer, Constanze; Graf, T. (Thomas) (Elsevier, 2015)
    Transcription-factor-induced somatic cell conversions are highly relevant for both basic and clinical research yet their mechanism is not fully understood and it is unclear whether they reflect normal differentiation ...
  • C/EBPα mediates the growth inhibitory effect of progestins on breast cancer cells
    Nacht, A. Silvina; Ferrari, Roberto; Zaurín Quer, Roser; Scabia, Valentina; Carbonell-Caballero, Jose; Le Dily, François; Quilez Oliete, Javier; Leopoldi, Alexandra; Brisken, Cathrin; Beato, Miguel; Vicent, Guillermo Pablo (EMBO Press, 2019)
    Steroid hormones are key gene regulators in breast cancer cells. While estrogens stimulate cell proliferation, progestins activate a single cell cycle followed by proliferation arrest. Here, we use biochemical and genome-wide ...
  • Chromatin topology defines estradiol-primed progesterone receptor and PAX2 binding in endometrial cancer cells
    La Greca, Alejandro; Bellora Pereyra, Nicolás; Le Dily, François; Jara, Rodrigo; Nacht, A. Silvina; Quilez Oliete, Javier; Villanueva Cañas, José Luis, 1984-; Vidal, Enric; Merino, Gabriela; Fresno, Cristóbal; Reischle, Inti Tarifa; Vallejo, Griselda; Vicent, Guillermo Pablo; Fernández, Elmer; Beato, Miguel; Saragüeta, Patricia (eLife Sciences Publications, 2022)
    Estrogen (E2) and Progesterone (Pg), via their specific receptors (ERalpha and PR), are major determinants in the development and progression of endometrial carcinomas, However, their precise mechanism of action and the ...
  • Hormone-control regions mediate steroid receptor-dependent genome organization
    Le Dily, François; Vidal, Enrique; Cuartero, Yasmina; Quilez Oliete, Javier; Nacht, A. Silvina; Vicent, Guillermo Pablo; Carbonell-Caballero, Jose; Sharma, Priyanka; Villanueva Cañas, José Luis, 1984-; Ferrari, Roberto; De Llobet, Lara Isabel; Verde, Gaetano; Wright, Roni H.G.; Beato, Miguel (Cold Spring Harbor Laboratory Press (CSHL Press), 2019)
    In breast cancer cells, some topologically associating domains (TADs) behave as hormonal gene regulation units, within which gene transcription is coordinately regulated in response to steroid hormones. Here we further ...
  • Nucleosome-driven transcription factor binding and gene regulation
    Ballaré, Cecilia Julia; Castellano, Giancarlo; Gaveglia, Laura; Althammer, Sonja Daniela; González-Vallinas Rostes, Juan, 1983-; Eyras Jiménez, Eduardo; Le Dily, François; Zaurín Quer, Roser; Soronellas, Daniel; Vicent, Guillermo Pablo; Beato, Miguel (Elsevier, 2013)
    In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by ...
  • Relationship between nucleosome positioning and progesterone-induced alternative splicing in breast cancer cells
    Iannone, Camilla, 1984-; Pohl, Andy, 1979-; Papasaikas, Panagiotis; Soronellas, Daniel; Vicent, Guillermo Pablo; Beato, Miguel; Valcárcel, J. (Juan) (Cold Spring Harbor Laboratory Press, 2015)
    Splicing of mRNA precursors can occur cotranscriptionally and it has been proposed that chromatin structure influences splice site recognition and regulation. Here we have systematically explored potential links between ...
  • Sequence-Dependent nucleosome positioning and chromatin remolleing of hormone-responsive genes
    Zaurín Quer, Roser (Universitat Pompeu Fabra, 2009-07-16)
    Evidències recents han remarcat la importància del paper de l'estructura de la cromatina i el posicionament de nucleosomes en processos cel·lulars bàsics com és la regulació de la transcripció gènica. L'avenç de noves ...
  • The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers
    Ceballos Chávez, María; Subtil Rodriguez, Alicia; Giannopoulou, Eugenia G.; Soronellas, Daniel; Vázquez Chávez, Elena; Vicent, Guillermo Pablo; Elemento, Olivier; Beato, Miguel; Reyes, José C. (Public Library of Science (PLoS), 2015)
    While the importance of gene enhancers in transcriptional regulation is well established, the mechanisms and the protein factors that determine enhancers activity have only recently begun to be unravelled. Recent studies ...
  • The glucocorticoid receptor interferes with progesterone receptor-dependent genomic regulation in breast cancer cells
    Ogara, Maria F.; Rodríguez Seguí, Santiago A.; Marini, Melisa; Silvina Nacht, Ana; Stortz, Martin; Levi, Valeria; Presman, Diego M.; Vicent, Guillermo Pablo; Pecci, Adali (Oxford University Press, 2019)
    The glucocorticoid and progesterone receptors (GR and PR) are closely related members of the steroid receptor family. Despite sharing similar structural and functional characteristics; the cognate hormones display very ...