Browsing by Author "Wieser, Stefan"

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  • A reconstituted mammalian APC-kinesin complex selectively transports defined packages of axonal mRNAs
    Baumann, Sebastian; Komissarov, Artem, 1992-; Gili, Maria; Ruprecht, Verena; Wieser, Stefan; Maurer, Sebastian (American Association for the Advancement of Science (AAAS), 2020)
    Through the asymmetric distribution of messenger RNAs (mRNAs), cells spatially regulate gene expression to create cytoplasmic domains with specialized functions. In neurons, mRNA localization is required for essential ...
  • Cooperative epithelial phagocytosis enables error correction in the early embryo
    Hoijman, Esteban; Häkkinen, Hanna-Maria; Tolosa-Ramon, Queralt; Jiménez-Delgado, Senda; Wyatt, Christopher Douglas Robert, 1988-; Miret-Cuesta, Marta; Irimia Martínez, Manuel; Callan-Jones, Andrew; Wieser, Stefan; Ruprecht, Verena (Nature Research, 2021)
    Errors in early embryogenesis are a cause of sporadic cell death and developmental failure1,2. Phagocytic activity has a central role in scavenging apoptotic cells in differentiated tissues3-6. However, how apoptotic cells ...
  • Mesoscale modeling and single-nucleosome tracking reveal remodeling of clutch folding and dynamics in stem cell differentiation
    Gómez García, Pablo; Portilllo-Ledesma, Stephanie; Neguembor, Maria Victoria; Pesaresi, Martina; Oweis, Walaa; Rohrlich, Talia; Wieser, Stefan; Meshorer, Eran; Schlick, Tamar; Cosma, Maria Pia; Lakadamyali, Melike (Science Direct, 2021)
    Nucleosomes form heterogeneous groups in vivo, named clutches. Clutches are smaller and less dense in mouse embryonic stem cells (ESCs) compared to neural progenitor cells (NPCs). Using coarse-grained modeling of the ...
  • Modulation-enhanced localization microscopy
    Reymond, Loïc; Huser, Thomas; Ruprecht, Verena; Wieser, Stefan (IOP Publishing Ltd., 2020)
    Super-resolution fluorescence microscopy has become a powerful tool in cell biology to observe sub-cellular organization and molecular details below the diffraction limit of light. Super-resolution methods are generally ...
  • Modulation-enhanced localization microscopy: using patterned illumination for super-resolution microscopy: from the localization of single molecules to the descripton of nano-structures
    Reymond, Loïc (Universitat Pompeu Fabra, 2023-09-26)
    This thesis introduces SIMPLE (Structured Illumination based Point Lo- calization Estimator), a novel super-resolution technique in fluorescence microscopy. By combining standing-wave patterned illumination with single-molecule ...
  • SIMPLE: structured illumination based point localization estimator with enhanced precision
    Reymond, Loïc; Ziegler, Johannes; Knapp, Christian; Wang, Fung-Chen; Huser, Thomas; Ruprecht, Verena; Wieser, Stefan (Optica, 2019)
    We present a structured illumination microscopy based point localization estimator (SIMPLE) that achieves a 2-fold increase in single molecule localization precision compared to conventional centroid estimation methods. ...
  • The nucleus measures shape changes for cellular proprioception to control dynamic cell behavior
    Venturini, Valeria; Pezzano, Fabio; Català Castro, Frederic; Häkkinen, Hanna-Maria; Jiménez-Delgado, Senda; Colomer-Rosell, Mariona; Marro, Monica; Tolosa-Ramon, Queralt; Paz-López, Sonia; Valverde, M. A. (Miguel Ángel), 1963-; Weghuber, Julian; Loza-Alvarez, Pablo; Krieg, Michael; Wieser, Stefan; Ruprecht, Verena (American Association for the Advancement of Science (AAAS), 2020)
    The physical microenvironment regulates cell behavior during tissue development and homeostasis. How single cells decode information about their geometrical shape under mechanical stress and physical space constraints ...

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