Browsing by Author "Selent, Jana"

Sort by: Order: Results:

  • A molecular sensor for cholesterol in the human serotonin 1A receptor
    Kumar, G Aditya; Sarkar, Parijat; Stepniewski, Tomasz Maciej, 1988-; Jafurulla, Md; Singh, Shishu Pal; Selent, Jana; Chattopadhyay, Amitabha (American Association for the Advancement of Science (AAAS), 2021)
    The function of several G protein-coupled receptors (GPCRs) exhibits cholesterol sensitivity. Cholesterol sensitivity of GPCRs could be attributed to specific sequence and structural features, such as the cholesterol ...
  • Allosteric modulation of GPCR-induced β-arrestin trafficking and signaling by a synthetic intrabody
    Baidya, Mithu; Chaturvedi, Madhu; Dwivedi, Hemlata; Ranjan, Ashutosh; Devost, Dominic; Namkung, Yoon; Stepniewski, Tomasz Maciej, 1988-; Pandey, Shubhi; Baruah, Minakshi; Panigrahi, Bhanupriya; Sarma, Parishmita; Yadav, Manish K.; Maharana, Jagannath; Banerjee, Ramanuj; Kawakami, Kouki; Inoue, Asuka; Selent, Jana; Laporte, Stéphane A.; Hébert, Terence E.; Shukla, Arun K. (Nature Research, 2022)
    Agonist-induced phosphorylation of G protein-coupled receptors (GPCRs) is a primary determinant of β-arrestin (βarr) recruitment and trafficking. For several GPCRs such as the vasopressin receptor subtype 2 (V2R), ...
  • C-edge loops of arrestin function as a membrane anchor
    Lally, Ciara C.M.; Bauer, Brain; Selent, Jana; Sommer, Martha E. (Nature Publishing Group, 2017)
    G-protein-coupled receptors are membrane proteins that are regulated by a small family of arrestin proteins. During formation of the arrestin–receptor complex, arrestin first interacts with the phosphorylated receptor C ...
  • Can molecular dynamics simulations improve the structural accuracy and virtual screening performance of GPCR models?
    Kapla, Jon; Rodríguez Espigares, Ismael, 1990-; Ballante, Flavio; Selent, Jana; Carlsson, Jens (Public Library of Science (PLoS), 2021)
    The determination of G protein-coupled receptor (GPCR) structures at atomic resolution has improved understanding of cellular signaling and will accelerate the development of new drug candidates. However, experimental ...
  • Community guidelines for GPCR ligand bias: IUPHAR review 32
    Kolb, Peter; Kenakin, Terry; Alexander, Stephen P. H.; Bermudez, Marcel; Bohn, Laura M.; Breinholt, Christian S.; Bouvier, Michel; Hill, Stephen J.; Kostenis, Evi; Martemyanov, Kirill A.; Neubig, Rick R.; Onaran, H. Ongun; Rajagopal, Sudarshan; Roth, Bryan L.; Selent, Jana; Shukla, Arun K.; Sommer, Martha E.; Gloriam, David E. (Wiley, 2022)
    GPCRs modulate a plethora of physiological processes and mediate the effects of one-third of FDA-approved drugs. Depending on which ligand activates a receptor, it can engage different intracellular transducers. This 'biased ...
  • Conformational sensors and domain swapping reveal structural and functional differences between β-arrestin isoforms
    Ghosh, Eshan; Dwivedi, Hemlata; Baidya, Mithu; Srivastava, Ashish; Kumari, Punita; Stepniewski, Tomek; Kim, Hee Ryung; Lee, Mi-Hye; van Gastel, Jaana; Chaturvedi, Madhu; Roy, Debarati; Pandey, Shubhi; Maharana, Jagannath; Guixà González, Ramon, 1978-; Luttrell, Louis M.; Chung, Ka Young; Dutta, Somnath; Selent, Jana; Shukla, Arun K. (Elsevier, 2019)
    Desensitization, signaling, and trafficking of G-protein-coupled receptors (GPCRs) are critically regulated by multifunctional adaptor proteins, β-arrestins (βarrs). The two isoforms of βarrs (βarr1 and 2) share a high ...
  • Development of fluorescent probes that target serotonin 5-HT2B receptors
    Azuaje, Jhonny; López, Paula; Iglesias, Alba; de la Fuente, Rocío Ailim; Pérez Rubio, José M.; García, Diego; Maciej Stępniewski, Tomasz; García Mera, Xerardo; Brea, José; Selent, Jana; Pérez Sala, Dolores; Castro, Marián; Loza, María I.; Sotelo, Eddy (Nature Publishing Group, 2017)
    Some 5-HT2B fluorescent probes were obtained by tagging 1-(2,5-dimethoxy-4-iodophenyl)-propan-2-amine (DOI) with a subset of fluorescent amines. Some of the resulting fluorescent ligands showed excellent affinity and ...
  • Discovery of a dual Ras and ARF6 inhibitor from a GPCR endocytosis screen
    Giubilaro, Jenna; Schuetz, Doris A.; Stepniewski, Tomasz Maciej, 1988-; Namkung, Yoon; Khoury, Etienne; Lara Márquez, Mónica; Campbell, Shirley; Beautrait, Alexandre; Armando, Sylvain; Radresa, Olivier; Duchaine, Jean; Lamarche Vane, Nathalie; Claing, Audrey; Selent, Jana; Bouvier, Michel; Marinier, Anne; Laporte, Stéphane A. (Nature Research, 2021)
    Internalization and intracellular trafficking of G protein-coupled receptors (GPCRs) play pivotal roles in cell responsiveness. Dysregulation in receptor trafficking can lead to aberrant signaling and cell behavior. Here, ...
  • Distinct phosphorylation sites in a prototypical GPCR differently orchestrate β-arrestin interaction, trafficking, and signaling
    Dwivedi, Hemlata; Chaturvedi, Madhu; Baidya, Mithu; Stepniewski, Tomasz Maciej, 1988-; Pandey, Shubhi; Maharana, Jagannath; Srivastava, Ashish; Caengprasath, Natarin; Hanyaloglu, Aylin C.; Selent, Jana; Shukla, Arun K. (American Association for the Advancement of Science (AAAS), 2020)
    Agonist-induced phosphorylation of G protein-coupled receptors (GPCRs) is a key determinant for their interaction with β-arrestins (βarrs) and subsequent functional responses. Therefore, it is important to decipher the ...
  • Dopamine D 2 receptor agonist binding kinetics-role of a conserved serine residue
    Ågren, Richard; Stepniewski, Tomasz Maciej, 1988-; Zeberg, Hugo; Selent, Jana; Sahlholm, Kristoffer (MDPI, 2021)
    The forward (kon) and reverse (koff) rate constants of drug-target interactions have important implications for therapeutic efficacy. Hence, time-resolved assays capable of measuring these binding rate constants may be ...
  • A dynamic view of molecular switch behavior at serotonin receptors: implications for functional selectivity
    Martí Solano, Maria; Sanz, Ferran; Pastor Maeso, Manuel; Selent, Jana (Public Library of Science (PLoS), 2014)
    Functional selectivity is a property of G protein-coupled receptors that allows them to preferentially couple to particular signaling partners upon binding of biased agonists. Publication of the X-ray crystal structure of ...
  • Electron paramagnetic resonance gives evidence for the presence of type 1 gonadotropin-releasing hormone receptor (GnRH-R) in subdomains of lipid rafts
    Koklič, Tilen; Hrovat, Alenka; Guixà González, Ramon, 1978-; Rodríguez Espigares, Ismael, 1990-; Navio, Damaris; Frangež, Robert; Uršič, Matjaž; Kubale, Valentina; Plemenitaš, Ana; Selent, Jana; Šentjurc, Marjeta; Vrecl, Milka (MDPI, 2021)
    This study investigated the effect of type 1 gonadotropin releasing hormone receptor (GnRH-R) localization within lipid rafts on the properties of plasma membrane (PM) nanodomain structure. Confocal microscopy revealed ...
  • Entrectinib-A SARS-CoV-2 inhibitor in Human Lung Tissue (HLT) cells
    Peralta-Garcia, Alejandro; Torrens Fontanals, Mariona; Stepniewski, Tomasz Maciej, 1988-; Grau-Expósito, Judith; Perea, David; Ayinampudi, Vikram; Waldhoer, Maria; Zimmermann, Mirjam; Buzón, María J.; Genescà, Meritxell; Selent, Jana (MDPI, 2021)
    Since the start of the COVID-19 outbreak, pharmaceutical companies and research groups have focused on the development of vaccines and antiviral drugs against SARS-CoV-2. Here, we apply a drug repurposing strategy to ...
  • Fractal dimension as a measure of surface roughness of G protein-coupled receptors: implications for structure and function
    Kaczor, Agnieszka A.; Guixà González, Ramon, 1978-; Carrió Gaspar, Pau, 1982-; Obiol Pardo, Cristian; Pastor Maeso, Manuel; Selent, Jana (Springer, 2012)
    Protein surface roughness is a structural property associated with ligand-protein and protein-protein binding interfaces. In this work we apply for the first time the concept of surface roughness, expressed as the fractal ...
  • GPCRmd - A web platform for collection, visualization and analysis of molecular dynamics data for G protein-coupled receptors : Bridging the gap between dynamics and receptor functionality
    Rodríguez Espigares, Ismael, 1990- (Universitat Pompeu Fabra, 2018-04-04)
    In this thesis, we present GPCRmd, an online repository with a submission system and visualization platform specifically designed for storing and providing easy access to molecular dynamics (MD) data of G protein-coupled ...
  • How do molecular dynamics data complement static structural data of GPCRs
    Torrens Fontanals, Mariona; Stepniewski, Tomasz Maciej, 1988-; Aranda García, David; Morales Pastor, Adrián; Medel Lacruz, Brian; Selent, Jana (MDPI, 2020)
    G protein-coupled receptors (GPCRs) are implicated in nearly every physiological process in the human body and therefore represent an important drug targeting class. Advances in X-ray crystallography and cryo-electron ...
  • In silico study of allosteric communication networks in GPCR signaling bias
    Morales Pastor, Adrián; Nerín-Fonz, Francho; Aranda García, David; Dieguez-Eceolaza, Miguel; Medel Lacruz, Brian; Torrens Fontanals, Mariona; Peralta-Garcia, Alejandro; Selent, Jana (MDPI, 2002)
    Signaling bias is a promising characteristic of G protein-coupled receptors (GPCRs) as it provides the opportunity to develop more efficacious and safer drugs. This is because biased ligands can avoid the activation of ...
  • Induced effects of sodium ions on dopaminergic G-protein coupled receptors
    Selent, Jana; Sanz, Ferran; Pastor Maeso, Manuel; De Fabritiis, Gianni (Public Library of Science (PLoS), 2010)
    G-protein coupled receptors, the largest family of proteins in the human genome, are involved in many complex signal transduction pathways, typically activated by orthosteric ligand binding and subject to allosteric ...
  • Ligand selectivity at GPCRs: from multitarget binding profiles to biased agonism
    Martí Solano, Maria (Universitat Pompeu Fabra, 2015-02-10)
    La caracterització de diversos fàrmacs capaços d’unir-se a receptors associats a proteïnes G (GPCR)s ha revelat que els seus mecanismes d’acció no eren tan simples com es creia. Primer, l’anàlisi de la seva afinitat d’unió ...
  • Ligand with two modes of interaction with the dopamine D2 receptor-an induced-fit mechanism of insurmountable antagonism
    Ågren, Richard; Zeberg, Hugo; Stepniewski, Tomasz Maciej, 1988-; Free, R. Benjamin; Reilly, Sean W.; Luedtke, Robert R.; Århem, Peter; Ciruela, Francisco; Sibley, David R.; Mach, Robert H.; Selent, Jana; Nilsson, Johanna; Sahlholm, Kristoffer (American Chemical Society (ACS), 2020)
    A solid understanding of the mechanisms governing ligand binding is crucial for rational design of therapeutics targeting the dopamine D2 receptor (D2R). Here, we use G protein-coupled inward rectifier potassium (GIRK) ...