Browsing by Author "Otonkoski, Timo"

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  • CRISPR activation enables high-fidelity reprogramming into human pluripotent stem cells
    Sokka, Joonas; Yoshihara, Masahito; Kvist, Jouni; Laiho, Laura; Warren, Andrew; Stadelmann, Christian; Jouhilahti, Eeva-Mari; Kilpinen, Helena; Balboa, Diego; Katayama, Shintaro; Kyttälä, Aija; Kere, Juha; Otonkoski, Timo; Weltner, Jere; Trokovic, Ras (Elsevier, 2022)
    Conventional reprogramming methods rely on the ectopic expression of transcription factors to reprogram somatic cells into induced pluripotent stem cells (iPSCs). The forced expression of transcription factors may lead to ...
  • Differentiating functional human islet-like aggregates from pluripotent stem cells
    Barsby, Tom; Ibrahim, Hazem; Lithovius, Väinö; Montaser, Hossam; Balboa, Diego; Vähäkangas, Eliisa; Chandra, Vikash; Saarimäki-Vire, Jonna; Otonkoski, Timo (Elsevier, 2022)
    We present here a robust and reliable protocol by which to differentiate pancreatic islet-like aggregates (SC-islets) from human pluripotent stem cells. The 7-stage protocol mimics developmental patterning factors that ...
  • Genome editing of human pancreatic beta cell models: problems, possibilities and outlook
    Balboa, Diego; Prasad, Rashmi B.; Groop, Leif; Otonkoski, Timo (Springer, 2019)
    Understanding the molecular mechanisms behind beta cell dysfunction is essential for the development of effective and specific approaches for diabetes care and prevention. Physiological human beta cell models are needed ...
  • Loss of MANF causes childhood-onset syndromic diabetes due to increased endoplasmic reticulum stress
    Montaser, Hossam; Patel, Kashyap A.; Balboa, Diego; Ibrahim, Hazem; Lithovius, Väinö; Näätänen, Anna; Chandra, Vikash; Demir, Korcan; Acar, Sezer; Ben-Omran, Tawfeg; Colclough, Kevin; Locke, Jonathan M; Wakeling, Matthew; Lindahl, Maria; Hattersley, Andrew T.; Saarimäki-Vire, Jonna; Otonkoski, Timo (American Diabetes Association, 2021)
    Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an endoplasmic reticulum (ER)-resident protein that plays a crucial role in attenuating ER stress responses. Although MANF is indispensable for the survival and ...
  • The type 1 diabetes gene TYK2 regulates β-cell development and its responses to interferon-α
    Chandra, Vikash; Ibrahim, Hazem; Halliez, Clémentine; Prasad, Rashmi B.; Vecchio, Federica; Dwivedi, Om Prakash; Kvist, Jouni; Balboa, Diego; Saarimäki-Vire, Jonna; Montaser, Hossam; Barsby, Tom; Lithovius, Väinö; Artner, Isabella; Gopalakrishnan, Swetha; Groop, Leif; Mallone, Roberto; Eizirik, Décio L.; Otonkoski, Timo (Nature Research, 2022)
    Type 1 diabetes (T1D) is an autoimmune disease that results in the destruction of insulin producing pancreatic β-cells. One of the genes associated with T1D is TYK2, which encodes a Janus kinase with critical roles in ...