Visualitza per autoria "Neguembor, Maria Victoria"

Ordena per: Ordre: Resultats:

  • A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells
    Martin, Laura; Vicario, Chiara; Castells García, Àlvaro, 1991-; Lakadamyali, Melike; Neguembor, Maria Victoria; Cosma, Maria Pia (Elsevier, 2021)
    Here, we describe three complementary microscopy-based approaches to quantify morphological changes of chromatin organization in cultured adherent cells: the analysis of the coefficient of variation of DNA, the measurement ...
  • CEBPA phase separation links transcriptional activity and 3D chromatin hubs
    Christou-Kent, Marie; Cuartero, Sergi; Garcia-Cabau, Carla; Ruehle, Julia; Naderi, Julian; Erber, Julia; Neguembor, Maria Victoria; Plana Carmona, Marcos, 1993-; Alcoverro-Bertran, Marc; Andrés Aguayo, Luisa de; Klonizakis, Antonios; Julià Vilella, Eric; Lynch, Cian J.; Serrano, Manuel; Hnisz, Denes; Salvatella, Xavier; Graf, T. (Thomas); Stik, Grégoire (Elsevier, 2023)
    Cell identity is orchestrated through an interplay between transcription factor (TF) action and genome architecture. The mechanisms used by TFs to shape three-dimensional (3D) genome organization remain incompletely ...
  • Cohesin controls X chromosome structure remodeling and X-reactivation during mouse iPSC-reprogramming
    Generoso, Serena Francesca, 1988-; Neguembor, Maria Victoria; Hershberg, Elliot A.; Sadreyev, Ruslan Ilyasovich; Kurimoto, Kazuki; Yabuta, Yukihiro; Ricci, Raffaele; Audergon, Pauline; Bauer, Moritz, 1987-; Saitou, Mitinori; Hochedlinger, Konrad; Beliveau, Brian J.; Cosma, Maria Pia; Lee, Jeannie T.; Payer, Bernhard (National Academy of Sciences, 2023)
    Reactivation of the inactive X chromosome is a hallmark epigenetic event during reprogramming of mouse female somatic cells to induced pluripotent stem cells (iPSCs). This involves global structural remodeling from a ...
  • Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading
    Porter, Hayley; Li, Yang; Neguembor, Maria Victoria; Beltran, Manuel; Varsally, Wazeer; Martin, Laura; Tavares Cornejo, Manuel; Pezić, Dubravka; Bhamra, Amandeep; Surinova, Silvia; Jenner, Richard G.; Cosma, Maria Pia; Hadjur, Suzana (eLife, 2023)
    Most studies of cohesin function consider the Stromalin Antigen (STAG/SA) proteins as core complex members given their ubiquitous interaction with the cohesin ring. Here, we provide functional data to support the notion ...
  • From research to rapid response: mass COVID-19 testing by volunteers at the Centre for Genomic Regulation
    Ghose, Ritobrata; Aranguren-Ibáñez, Álvaro; Arecco, Niccolò; Balboa, Diego; Bataller, Marc; Beltran, Sergi; Benisty, Hannah, 1986-; Bénard, Angèle; Bernardo, Edgar; Carbonell Sala, Silvia; Casals, Eloi; Ciampi, Ludovica; Condemi, Livia; Corvò, Alberto; Cosín-Tomás, Marta; Cuenca-Ardura, Mirabai; Duran Serrano, Juan Manuel; Espejo Díaz, María Isabel; Fernández Callejo, Marcos; Gañez-Zapater, Antoni; Garcia-Castellanos, Raquel; Garrido, Romina; Henkin, Gil; Hermoso Pulido, Antonio; Hernandez-Alias, Xavier; Herrero Vicente, Jorge; Ingham, Matthew; Lim, Wei Ming; Llonch, Sílvia; Marmesat Bertoli, Elena; Miguel Escalada, Irene; Montero-Blay, Ariadna; Navarrete Hernández, Cristina; Neguembor, Maria Victoria; Ní Chárthaigh, Róisín-Ana; Pardo-Lorente, Natalia; Pascual-Reguant, Laura, 1990-; Pérez-Lluch, Sílvia; Perza, Reyes; Pesaresi, Martina, 1991-; Picó Amado, Daniel; Pifarré, Paula; Piscia, Davide; Plana-Carmona, Marcos; Ponomarenko, Julia; Radusky, Leandro; Rivero, Ezequiel; Rogalska, Malgorzata; Torcal Garcia, Guillem, 1991-; Wojnacki, José (F1000Research, 2020)
    The COVID-19 pandemic has posed and is continuously posing enormous societal and health challenges worldwide. The research community has mobilized to develop novel projects to find a cure or a vaccine, as well as to ...
  • Mesoscale modeling and single-nucleosome tracking reveal remodeling of clutch folding and dynamics in stem cell differentiation
    Gómez García, Pablo; Portilllo-Ledesma, Stephanie; Neguembor, Maria Victoria; Pesaresi, Martina; Oweis, Walaa; Rohrlich, Talia; Wieser, Stefan; Meshorer, Eran; Schlick, Tamar; Cosma, Maria Pia; Lakadamyali, Melike (Science Direct, 2021)
    Nucleosomes form heterogeneous groups in vivo, named clutches. Clutches are smaller and less dense in mouse embryonic stem cells (ESCs) compared to neural progenitor cells (NPCs). Using coarse-grained modeling of the ...
  • (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes
    Neguembor, Maria Victoria; Sebastián Pérez, Rubén; Aulicino, Francesco, 1987-; Gómez García, Pablo; Cosma, Maria Pia, 1970-; Lakadamyali, Melike (Oxford University Press, 2018)
    CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization ...
  • SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state
    Sebastián Pérez, Rubén; Nakagawa, Shoma; Tu, Xiaochuan; Aranda Aragón, Sergio; Pesaresi, Martina; Gómez García, Pablo; Alcoverro-Bertran, Marc; Gómez Vázquez, José Luis; Carnevali, Davide; Borràs, Eva; Sabidó Aguadé, Eduard, 1981-; Martin, Laura; Nissim Rafinia, Malka; Meshorer, Eran; Neguembor, Maria Victoria; Di Croce, Luciano; Cosma, Maria Pia (eLife, 2023)
    Chromocenters are established after the 2-cell (2C) stage during mouse embryonic development, but the factors that mediate chromocenter formation remain largely unknown. To identify regulators of 2C heterochromatin ...
  • Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches
    Castells García, Àlvaro, 1991-; Ed-Daoui, Ilyas; González-Almela, Esther; Vicario, Chiara; Ottestrom, Jason; Lakadamyali, Melike; Neguembor, Maria Victoria; Cosma, Maria Pia (Oxford University Press, 2022)
    Transcription and genome architecture are interdependent, but it is still unclear how nucleosomes in the chromatin fiber interact with nascent RNA, and which is the relative nuclear distribution of these RNAs and elongating ...
  • The magic of unraveling genome architecture and function
    Cosma, Maria Pia; Neguembor, Maria Victoria (Elsevier, 2023)
    Over the last decades, technological breakthroughs in super-resolution microscopy have allowed us to reach molecular resolution and design experiments of unprecedented complexity. Investigating how chromatin is folded in ...
  • The Suv420h histone methyltransferases regulate PPAR-γ and energy expenditure in response to environmental stimuli
    Pedrotti, Simona; Caccia, Roberta; Neguembor, Maria Victoria; García Manteiga, José Manuel; Ferri, Giulia; Palma, Clara de; Canu, Tamara; Giovarelli, Matteo; Marra, Paolo; Fiocchi, Amleto; Molineris, Ivan; Raso, Michele; Sanvito, Francesca; Doglioni, Claudio; Esposito, Antonio; Clementi, Emilio; Gabellini, Davide (American Association for the Advancement of Science (AAAS), 2019)
    Obesity and its associated metabolic abnormalities have become a global emergency with considerable morbidity and mortality. Epidemiologic and animal model data suggest an epigenetic contribution to obesity. Nevertheless, ...
  • Transcription-mediated supercoiling regulates genome folding and loop formation
    Neguembor, Maria Victoria; Martin, Laura; Castells García, Àlvaro, 1991-; Gómez García, Pablo; Vicario, Chiara; Carnevali, Davide; Abed, Jumana AlHaj; Granados, Alba; Sebastián Pérez, Rubén; Sottile, Francesco, 1988-; Solon, Jérôme; Wu, Chao-Ting; Lakadamyali, Melike; Cosma, Maria Pia, 1970- (Elsevier, 2021)
    The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin ...
  • Wnt/β-catenin signaling pathway safeguards epigenetic stability and homeostasis of mouse embryonic stem cells
    Theka, Ilda, 1984-; Sottile, Francesco, 1988-; Cammisa, Marco; Bonnin, Sarah; Sanchez-Delgado, Marta; Di Vicino, Umberto; Neguembor, Maria Victoria; Arumugam, Karthik; Aulicino, Francesco, 1987-; Monk, David; Riccio, Andrea; Cosma, Maria Pia, 1970- (Nature Research, 2019)
    Mouse embryonic stem cells (mESCs) are pluripotent and can differentiate into cells belonging to the three germ layers of the embryo. However, mESC pluripotency and genome stability can be compromised in prolonged in vitro ...
  • Women's contribution in understanding how topoisomerases, supercoiling, and transcription control genome organization
    Martin, Laura; Neguembor, Maria Victoria; Cosma, Maria Pia, 1970- (Frontiers, 2023)
    One of the biggest paradoxes in biology is that human genome is roughly 2 m long, while the nucleus containing it is almost one million times smaller. To fit into the nucleus, DNA twists, bends and folds into several ...

Cerca

Visualitza

El meu compte

Amb col·laboració de Complim Participem