Browsing by Author "Malhotra, Vivek"

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  • A physical mechanism of TANGO1-mediated bulky cargo export
    Raote, Ishier; Chabanon, Morgan; Walani, Nikhil; Arroyo, Marino; Garcia-Parajo, Maria F.; Malhotra, Vivek; Campelo, Felix (eLife, 2020)
    The endoplasmic reticulum (ER)-resident protein TANGO1 assembles into a ring around ER exit sites (ERES), and links procollagens in the ER lumen to COPII machinery, tethers, and ER-Golgi intermediate compartment (ERGIC) ...
  • ADF/cofilin regulates secretory cargo sorting at the TGN via the Ca2+ ATPase SPCA1
    Blume, Julia von; Alleaume, Anne-Marie; Cantero Recasens, Gerard, 1984-; Curwin, Amy; Carreras Sureda, Amado, 1986-; Zimmermann, Timo; Galen, Josse van; Wakana, Yuichi; Valverde, M. A. (Miguel Ángel), 1963-; Malhotra, Vivek (Elsevier, 2011)
    Actin-severing proteins ADF/cofilin are required for the sorting of secretory cargo at the trans-Golgi network (TGN) in mammalian cells. How do these cytoplasmic proteins interact with the cargoes in the lumen of the TGN? ...
  • Biallelic TANGO1 mutations cause a novel syndromal disease due to hampered cellular collagen secretion
    Lekszas, Caroline; Foresti, Ombretta; Raote, Ishier; Liedtke, Daniel; König, Eva Maria; Nanda, Indrajit; Vona, Barbara; Coster, Peter de; Cauwels, Rita; Malhotra, Vivek; Haaf, Thomas (eLife, 2020)
    The transport and Golgi organization 1 (TANGO1) proteins play pivotal roles in the secretory pathway. Full length TANGO1 is a transmembrane protein localised at endoplasmic reticulum (ER) exit sites, where it binds bulky ...
  • Cab45 is required for Ca(2+)-dependent secretory cargo sorting at the trans-Golgi network
    Blume, Julia von; Alleaume, Anne-Marie; Kienzle, Christine; Carreras Sureda, Amado, 1986-; Valverde, M. A. (Miguel Ángel), 1963-; Malhotra, Vivek (Rockefeller University Press, 2012)
    Ca(2+) import into the lumen of the trans-Golgi network (TGN) by the secretory pathway calcium ATPase1 (SPCA1) is required for the sorting of secretory cargo. How is Ca(2+) retained in the lumen of the Golgi, and what is ...
  • Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast
    Curwin, Amy; Blume, Julia von; Malhotra, Vivek (American Society for Cell Biology, 2012)
    The mechanism of cargo sorting at the trans-Golgi network (TGN) for secretion is poorly understood. We previously reported the involvement of the actin-severing protein cofilin and the Ca(2+) ATPase secretory pathway calcium ...
  • Discovery of putative prognostic and therapeutic miRNA in uEVs of Dent's Disease 1 patients and characterisation of cellular models of the disease
    Burballa Tàrrega, Carla, 1988- (2021-05-14)
    Dent disease 1 (DD1) is a rare renal tubulopathy caused by CLCN5 mutations and characterized by low molecular weight proteinuria, variable hypercalciuria, nephrocalcinosis and/or nephrolithiasis and progression to kidney ...
  • Editorial: Unconventional protein secretion: From basic mechanisms to dysregulation in disease
    Chiritoiu, Marioara; Stewart, Sarah E.; Zhang, Min; Malhotra, Vivek; Villeneuve, Julien (Frontiers, 2022)
  • ESCRT-III drives the final stages of CUPS maturation for unconventional protein secretion
    Curwin, Amy; Brouwers, Nathalie; Alonso Y Adell, Manuel; Teis, David; Turacchio, Gabriele; Parashuraman, Seetharaman; Ronchi, Paolo; Malhotra, Vivek (eLife, 2016)
    The unconventional secretory pathway exports proteins that bypass the endoplasmic reticulum. In Saccharomyces cerevisiae, conditions that trigger Acb1 secretion via this pathway generate a Grh1 containing compartment ...
  • GRASP55 and UPR control interleukin-1β aggregation and secretion
    Chiritoiu, Marioara; Brouwers, Nathalie; Turacchio, Gabriele; Pirozzi, Marianella; Malhotra, Vivek (Elsevier, 2019)
    Signal-sequence-lacking interleukin (IL)-1β, is cleaved by caspase-1 to mature mIL-1β, which is secreted, without entering the endoplasmic reticulum. We report that macrophages of GRASP55-/- mice are defective in mIL-1β ...
  • GRh1-dependent unconventional protein secretion
    Bruns, Caroline, 1984- (Universitat Pompeu Fabra, 2013-04-30)
    A part de la secreció convencional, en la qual les proteïnes són transportades a través del reticle endoplasmàtic (RE) i de l'aparell de Golgi, s'han descrit, per a diferents proteïnes i en organismes diversos, rutes de ...
  • KChIP3 coupled to Ca2+ oscillations exerts a tonic brake on baseline mucin release in the colon
    Cantero Recasens, Gerard, 1984-; Butnaru, Cristian M.; Valverde, M. A. (Miguel Ángel), 1963-; Naranjo, José R.; Brouwers, Nathalie; Malhotra, Vivek (eLife, 2018)
    Regulated mucin secretion from specialized goblet cells by exogenous agonist-dependent (stimulated) and -independent (baseline) manner is essential for the function of the epithelial lining. Over extended periods, baseline ...
  • Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface
    Wakana, Yuichi; Villeneuve, Julien; Galen, Josse van; Cruz Garcia, David; Tagaya, Mitsuo; Malhotra, Vivek (Rockefeller University Press, 2013)
    Here we report that the kinesin-5 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for protein transport from the Golgi complex to the cell surface in Drosophila S2 cells. ...
  • New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells
    Bassaganyas Bars, Laia, 1985-; Popa, Stephanie J.; Horlbeck, Max; Puri, Claudia; Stewart, Sarah E.; Campelo, Felix; Ashok, Anupama, 1985-; Butnaru, Cristian M.; Brouwers, Nathalie; Heydari, Kartoosh; Ripoche, Jean; Weissman, Jonathan; Rubinsztein, David C.; Schekman, Randy; Malhotra, Vivek; Moreau, Kevin; Villeneuve, Julien (Rockefeller University Press, 2019)
    Protein and membrane trafficking pathways are critical for cell and tissue homeostasis. Traditional genetic and biochemical approaches have shed light on basic principles underlying these processes. However, the list of ...
  • Novel Dent disease 1 cellular models reveal biological processes underlying ClC-5 loss-of-function
    Durán, Mónica; Burballa Tàrrega, Carla, 1988-; Cantero Recasens, Gerard, 1984-; Butnaru, Cristian M.; Malhotra, Vivek; Ariceta, Gema; Sarró, Eduard; Meseguer, Anna (Oxford University Press, 2021)
    Dent disease 1 (DD1) is a rare X-linked renal proximal tubulopathy characterized by low molecular weight proteinuria and variable degree of hypercalciuria, nephrocalcinosis and/or nephrolithiasis, progressing to chronic ...
  • Protein transport by vesicles and tunnels
    Raote, Ishier; Malhotra, Vivek (Rockefeller University Press, 2019)
    Palade's corpus placed small vesicles as the sole means to transport proteins across stable distinct compartments of the secretory pathway. We suggest that cargo, spatial organization of secretory compartments, and the ...
  • Reactive oxygen species triggers unconventional secretion of antioxidants and Acb1
    Cruz Garcia, David; Brouwers, Nathalie; Malhotra, Vivek; Curwin, Amy (Rockefeller University Press, 2020)
    Nutrient deprivation triggers the release of signal-sequence-lacking Acb1 and the antioxidant superoxide dismutase 1 (SOD1). We now report that secreted SOD1 is functionally active and accompanied by export of other ...
  • Reversing chemorefraction in colorectal cancer cells by controlling mucin secretion
    Cantero Recasens, Gerard, 1984-; Alonso-Marañón, Josune; Lobo-Jarne, Teresa; Garrido, Marta; Iglesias Coma, Mar; Espinosa Blay, Lluís; Malhotra, Vivek (eLife, 2022)
    Fifteen percent of colorectal cancer (CRC) cells exhibit a mucin hypersecretory phenotype, which is suggested to provide resistance to immune surveillance and chemotherapy. We now formally show that CRC cells build a barrier ...
  • Sodium channel TRPM4 and sodium/calcium exchangers (NCX) cooperate in the control of Ca2+-induced mucin secretion from goblet cells
    Cantero Recasens, Gerard, 1984-; Butnaru, Cristian M.; Brouwers, Nathalie; Mitrovic, Sandra; Valverde, M. A. (Miguel Ángel), 1963-; Malhotra, Vivek (American Society for Biochemistry and Molecular Biology (ASBMB), 2019)
    Regulated mucin secretion is essential for the formation of the mucus layer that protects the underlying epithelial cells from foreign particles. Alterations in the quantity or quality of secreted mucins are therefore ...
  • Sphingomyelin metabolism controls the shape and function of the Golgi cisternae
    Campelo, Felix; van Galen, Josse; Turacchio, Gabriele; Parashuraman, Seetharaman; Kozlov, Michael M.; García Parajó, María F.; Malhotra, Vivek (eLife, 2017)
    The flat Golgi cisterna is a highly conserved feature of eukaryotic cells, but how is this morphology achieved and is it related to its function in cargo sorting and export? A physical model of cisterna morphology led us ...
  • TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulum
    Santos, António J. M.; Nogueira, Cristina; Ortega Bellido, María; Malhotra, Vivek (Rockefeller University Press, 2016)
    Procollagens, pre-chylomicrons, and pre–very low-density lipoproteins (pre-VLDLs) are too big to fit into conventional COPII-coated vesicles, so how are these bulky cargoes exported from the endoplasmic reticulum (ER)? We ...