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Browsing by Author "Lucas, Morghan C."

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Accurate detection of m6A RNA modifications in native RNA sequences

Liu, Huanle; Begik, Oguzhan; Lucas, Morghan C.; Ramirez, Jose Miguel; Mason, Christopher E.; Wiener, David; Schwartz, Schraga; Mattick, John S.; Smith, Martin A.; Novoa, Eva Maria (Nature Research, 2019)

The epitranscriptomics field has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here, we show that using direct ...
Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart

Milenkovic, Ivan; Santos Vieira, Helaine Graziele; Lucas, Morghan C.; Ruiz-Orera, Jorge; Patone, Giannino; Kesteven, Scott; Wu, Jianxin; Feneley, Michael; Espadas, Guadalupe; Sabidó Aguadé, Eduard, 1981-; Hübner, Norbert; van Heesch, Sebastiaan; Völkers, Mirko; Novoa, Eva Maria (Oxford University Press, 2023)

The existence of naturally occurring ribosome heterogeneity is now a well-acknowledged phenomenon. However, whether this heterogeneity leads to functionally diverse 'specialized ribosomes' is still a controversial topic. ...
Enhanced detection of RNA modifications and read mapping with high-accuracy nanopore RNA basecalling models

Diensthuber, Gregor; Pryszcz, Leszek Piotr, 1985-; Llovera Nadal, Laia; Lucas, Morghan C.; Delgado-Tejedor, Anna; Cruciani, Sonia; Roignant, Jean-Yves; Begik, Oguzhan; Novoa, Eva Maria (Cold Spring Harbor Laboratory Press (CSHL Press), 2024)

In recent years, nanopore direct RNA sequencing (DRS) became a valuable tool for studying the epitranscriptome, due to its ability to detect multiple modifications within the same full-length native RNA molecules. While ...
High-performance nano-flow liquid chromatography column combined with high- and low-collision energy data-independent acquisition enables targeted and discovery identification of modified ribonucleotides by mass spectrometry

Espadas, Guadalupe; Morales Sanfrutos, Julia; Medina, Rebeca; Lucas, Morghan C.; Novoa, Eva Maria; Sabidó Aguadé, Eduard, 1981- (Elsevier, 2022)

Over 170 post-transcriptional RNA modifications have been described and are common in all kingdoms of life. These modifications range from methylation to complex chemical structures, with methylation being the most abundant. ...
Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures

Begik, Oguzhan; Lucas, Morghan C.; Liu, Huanle; Ramírez, José Miguel; Mattick, John S.; Novoa, Eva Maria (BioMed Central, 2020)

Background: RNA modifications play central roles in cellular fate and differentiation. However, the machinery responsible for placing, removing, and recognizing more than 170 RNA modifications remains largely uncharacterized ...
Investigating sperm RNA dynamics in intergenerational inheritance using nanopore sequencing

Lucas, Morghan C. (Universitat Pompeu Fabra, 2023-05-03)

The aim of this thesis is to delve into sperm RNA dynamics during maturation and paternal inheritance. To obtain an integrative multi-omics view of sperm RNA during these processes, I turned to the new direct RNA sequencing ...
Molecular barcoding of native RNAs using nanopore sequencing and deep learning

Smith, Martin A.; Ersavas, Tansel; Ferguson, James M.; Liu, Huanle; Lucas, Morghan C.; Begik, Oguzhan; Bojarski, Lilly; Barton, Kirston; Novoa, Eva Maria (Cold Spring Harbor Laboratory Press (CSHL Press), 2020)

Nanopore sequencing enables direct measurement of RNA molecules without conversion to cDNA, thus opening the gates to a new era for RNA biology. However, the lack of molecular barcoding of direct RNA nanopore sequencing ...
Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing

Lucas, Morghan C.; Pryszcz, Leszek Piotr, 1985-; Medina, Rebeca; Milenkovic, Ivan; Camacho, Noelia; Marchand, Virginie; Motorin, Yuri; Ribas de Pouplana, Lluís; Novoa, Eva Maria (Nature Research, 2024)

Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been difficult in part because a simple method to simultaneously quantify their abundance and chemical modifications is lacking. Here we ...
Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing

Begik, Oguzhan; Lucas, Morghan C.; Pryszcz, Leszek Piotr, 1985-; Ramirez, Jose Miguel; Medina, Rebeca; Milenkovic, Ivan; Cruciani, Sonia; Liu, Huanle; Santos Vieira, Helaine Graziele; Sas Chen, Aldema; Mattick, John S.; Schwartz, Schraga; Novoa, Eva Maria (Nature Research, 2021)

Nanopore RNA sequencing shows promise as a method for discriminating and identifying different RNA modifications in native RNA. Expanding on the ability of nanopore sequencing to detect N6-methyladenosine, we show that ...
Temporally resolved proteomics identifies nidogen-2 as a cotarget in pancreatic cancer that modulates fibrosis and therapy response

Pereira, Brooke A.; Lucas, Morghan C.; Timpson, Paul (American Association for the Advancement of Science (AAAS), 2024)

Pancreatic ductal adenocarcinoma (PDAC) is characterized by increasing fibrosis, which can enhance tumor progression and spread. Here, we undertook an unbiased temporal assessment of the matrisome of the highly metastatic ...

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Browsing by Author "Lucas, Morghan C."

Browsing by Author "Lucas, Morghan C."

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