Listar por autoría "Lluch-Senar, Maria 1982-"

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  • A RAGE based strategy for the genome engineering of the human respiratory pathogen mycoplasma pneumoniae
    García Morales, Luis; Ruiz, Estelle; Gourgues, Géraldine; Rideau, Fabien; Piñero-Lambea, Carlos; Lluch-Senar, Maria 1982-; Blanchard, Alain; Lartigue, Carole (American Chemical Society (ACS), 2020)
    Genome engineering of microorganisms has become a standard in microbial biotechnologies. Several efficient tools are available for the genetic manipulation of model bacteria such as Escherichia coli and Bacillus subtilis, ...
  • Assessing the hodgepodge of non-mapped reads in bacterial transcriptomes: real or artifactual RNA chimeras?
    Lloréns Rico, Verónica, 1989-; Serrano Pubull, Luis, 1982-; Lluch-Senar, Maria 1982- (BioMed Central, 2014)
    Background: RNA sequencing methods have already altered our view of the extent and complexity of bacterial and eukaryotic transcriptomes, revealing rare transcript isoforms (circular RNAs, RNA chimeras) that could play an ...
  • Bacterial antisense RNAs are mainly the product of transcriptional noise
    Lloréns Rico, Verónica, 1989-; Cano, Jaime; Kamminga, Tjerko; Gil, Rosario; Latorre, Amparo; Chen, Wei-Hua; Bork, Peer; Glass, John I.; Serrano Pubull, Luis, 1982-; Lluch-Senar, Maria 1982- (American Association for the Advancement of Science (AAAS), 2016)
    cis-Encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of most of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the ...
  • Bacterial expression of a designed single-chain IL-10 prevents severe lung inflammation
    Montero-Blay, Ariadna, 1994-; Delgado Blanco, Javier; Rodriguez-Arce, Irene; Lastrucci, Claire; Piñero-Lambea, Carlos; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982- (EMBO Press, 2023)
    Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is active as a swapped domain dimer and is used in bacterial therapy of gut inflammation. IL-10 can be used as treatment of a wide range of pulmonary diseases. ...
  • Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms
    Blanco Cabra, Núria; Paetzold, Bernhard, 1981-; Ferrar, Tony; Mazzolini, Rocco; Torrents, Eduard; Serrano Pubull, Luis, 1982-; Lluch-Senar, Maria 1982- (Nature Research, 2020)
    Aggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as biofilms, are associated with several infectious diseases. One of the main components of ...
  • Comparative "-omics" in mycoplasma pneumoniae clinical isolates reveals key virulence factors
    Lluch-Senar, Maria 1982-; Cozzuto, Luca; Cano, Jaime; Delgado Blanco, Javier; Lloréns Rico, Verónica, 1989-; Pereyre, Sabine; Bebear, Cécile; Serrano Pubull, Luis, 1982- (Public Library of Science (PLoS), 2015)
    The human respiratory tract pathogen M. pneumoniae is one of the best characterized minimal bacterium. Until now, two main groups of clinical isolates of this bacterium have been described (types 1 and 2), differing in the ...
  • Comprehensive methylome characterization of Mycoplasma genitalium and Mycoplasma pneumoniae at single-base resolution
    Lluch-Senar, Maria 1982-; Luong, Khai; Lloréns Rico, Verónica, 1989-; Delgado Blanco, Javier; Fang, Gang; Spittle, Kristi; Clark, Tyson A.; Schadt, Eric; Turner, Stephen W.; Korlach, Jonas; Serrano Pubull, Luis, 1982- (Public Library of Science (PLoS), 2013)
    In the bacterial world, methylation is most commonly associated with restriction-modification systems that provide a defense mechanism against invading foreign genomes. In addition, it is known that methylation plays ...
  • Comprehensive quantitative modeling of translation efficiency in a genome-reduced bacterium
    Weber, Marc; Sogues, Adrià; Yus, Eva; Burgos, Raul; Gallo López, Carolina, 1984-; Martínez, Sira; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982- (EMBO Press, 2023)
    Translation efficiency has been mainly studied by ribosome profiling, which only provides an incomplete picture of translation kinetics. Here, we integrated the absolute quantifications of tRNAs, mRNAs, RNA half-lives, ...
  • CReasPy-Cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system.
    Ruiz, Estelle; Talenton, Vincent; Dubrana, Marie-Pierre; Guesdon, Gabrielle; Lluch-Senar, Maria 1982-; Salin, Franck; Sirand-Pugnet, Pascal; Arfi, Yonathan; Lartigue, Carole (American Chemical Society (ACS), 2019)
    Over the past decade, a new strategy was developed to bypass the difficulties to genetically engineer some microbial species by transferring (or "cloning") their genome into another organism that is amenable to efficient ...
  • Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
    Trussart, Marie, 1985-; Yus, Eva; Martínez, Sira; Baù, Davide; Tahara, Yuhei O.; Pengo, Thomas; Widjaja, Michael; Kretschmer, Simon; Swoger, Jim; Djordjevic, Steven P.; Turnbull, Lynne; Whitchurch, Cynthia B.; Miyata, Makoto; Martí Renom, Marc A.; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982- (Nature Publishing Group, 2017)
    DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional ...
  • Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium
    Lluch-Senar, Maria 1982-; Delgado Blanco, Javier; Chen, Wei-Hua; Lloréns Rico, Verónica, 1989-; O’Reilly, Francis J.; Wodke, Judith A.H.; Unal, E. Besray; Yus, Eva; Martínez, Sira; Nichols, Robert J.; Ferrar, Tony; Vivancos Prellezo, Ana; Schmeisky, Arne; Stülke, Jörg; Noort, Vera van; Gavin, Anne-Claude; Bork, Peer; Serrano Pubull, Luis, 1982- (Wiley Blackwell, 2015)
    Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found that small ORFs (smORFs; < 100 residues), accounting for ...
  • Determination of the gene regulatory network of a genome-reduced bacterium highlights alternative regulation independent of transcription factors
    Yus, Eva; Lloréns Rico, Verónica, 1989-; Martínez, Sira; Gallo López, Carolina, 1984-; Eilers, Hinnerk; Blötz, Cedric; Stülke, Jörg; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982- (Elsevier, 2019)
    Here, we determined the relative importance of different transcriptional mechanisms in the genome-reduced bacterium Mycoplasma pneumoniae, by employing an array of experimental techniques under multiple genetic and ...
  • Development of a serum-free medium to aid large-scale production of Mycoplasma-based therapies
    Burgos, Raul; Garcia Ramallo, Eva; Shaw, Daniel, 1993-; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982- (American Society for Microbiology, 2023)
    To assist in the advancement of the large-scale production of safe Mycoplasma vaccines and other Mycoplasma-based therapies, we developed a culture medium free of animal serum and other animal components for Mycoplasma ...
  • Development of computational and experimental tools for the identification of small proteins in bacterial genomes
    Miravet Verde, Samuel (2021-05-28)
    Small proteins (SEPs; <100aa) are involved in essential processes such as cell homeostasis, signalling, or metabolism. However, they have been overlooked because of computational and experimental difficulties that prevent ...
  • Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling
    Wodke, Judith A.H.; Puchalka, Jacek; Lluch-Senar, Maria 1982-; Marcos del Águila, Josep, 1971-; Yus, Eva; Godinho, Miguel; Gutiérrez Gallego, Ricardo, 1968-; Martins dos Santos, Vitor; Serrano Pubull, Luis, 1982-; Klipp, E., 1965-; Maier, Tobias (Nature Publishing Group, 2013)
    Mycoplasma pneumoniae, a threatening pathogen with a minimal genome, is a model organism for bacterial systems biology for which substantial experimental information is available. With the goal of understanding the complex ...
  • Distinguishing between productive and abortive promoters using a random forest classifier in Mycoplasma pneumoniae
    Lloréns Rico, Verónica, 1989-; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982- (Oxford University Press, 2015)
    Distinguishing between promoter-like sequences in bacteria that belong to true or abortive promoters, or to those that do not initiate transcription at all, is one of the important challenges in transcriptomics. To address ...
  • Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms
    Mazzolini, Rocco; Rodríguez-Arce, Irene; Fernández-Barat, Laia; Piñero-Lambea, Carlos; Garrido, Victoria; Rebollada-Merino, Agustín; Motos, Ana; Torres, Antoni; Grilló, María-Jesús; Serrano Pubull, Luis, 1982-; Lluch-Senar, Maria 1982- (Nature Research, 2023)
    Engineered live bacteria could provide a new modality for treating lung infections, a major cause of mortality worldwide. In the present study, we engineered a genome-reduced human lung bacterium, Mycoplasma pneumoniae, ...
  • Engineering a genome-reduced bacterium to eliminate Staphylococcus aureus biofilms in vivo
    Garrido, Victoria; Piñero-Lambea, Carlos; Rodriguez-Arce, Irene; Paetzold, Bernhard, 1981-; Ferrar, Tony; Weber, Marc; Garcia Ramallo, Eva; Gallo López, Carolina, 1984-; Collantes, María; Peñuelas, Iván; Serrano Pubull, Luis, 1982-; Grilló, María-Jesús; Lluch-Senar, Maria 1982- (Wiley Open Access, 2021)
    Bacteria present a promising delivery system for treating human diseases. Here, we engineered the genome-reduced human lung pathogen Mycoplasma pneumoniae as a live biotherapeutic to treat biofilm-associated bacterial ...
  • FASTQINS and ANUBIS: two bioinformatic tools to explore facts and artifacts in transposon sequencing and essentiality studies
    Miravet Verde, Samuel; Burgos, Raul; Delgado Blanco, Javier; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982- (Oxford University Press, 2020)
    Transposon sequencing is commonly applied for identifying the minimal set of genes required for cellular life; a major challenge in fields such as evolutionary or synthetic biology. However, the scientific community has ...
  • Functional characterization of the cell division gene cluster of the wall-less bacterium mycoplasma genitalium
    Martínez-Torró, Carlos; Torres-Puig, Sergi; Marcos-Silva, Marina; Huguet-Ramón, Marta; Muñoz-Navarro, Carmen; Lluch-Senar, Maria 1982-; Serrano Pubull, Luis, 1982-; Querol, Enrique; Piñol, Jaume; Pich, Oscar Q. (Frontiers Media, 2021)
    It is well-established that FtsZ drives peptidoglycan synthesis at the division site in walled bacteria. However, the function and conservation of FtsZ in wall-less prokaryotes such as mycoplasmas are less clear. In the ...

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