Browsing by Author "Gómez García, Pablo"

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  • Distinct properties and functions of CTCF revealed by a rapidly inducible degron system
    Luan, Jing; Xiang, Guanjue; Gómez García, Pablo; Tome, Jacob M.; Zhang, Zhe; Vermut, Marit W.; Zhang, Haoyue; Huang, Anran; Keller, Cheryl A.; Giardine, Belinda M.; Zhang, Yu; Lan, Yemin; Lis, John T.; Lakadamyali, Melike; Hardison, Ross C.; Blobel, Gerd A. (Cell Press, 2021)
    CCCTC-binding factor (CTCF) is a conserved zinc finger transcription factor implicated in a wide range of functions, including genome organization, transcription activation, and elongation. To explore the basis for CTCF ...
  • Mesoscale modeling and single-nucleosome tracking reveal remodeling of clutch folding and dynamics in stem cell differentiation
    Gómez García, Pablo; Portilllo-Ledesma, Stephanie; Neguembor, Maria Victoria; Pesaresi, Martina; Oweis, Walaa; Rohrlich, Talia; Wieser, Stefan; Meshorer, Eran; Schlick, Tamar; Cosma, Maria Pia; Lakadamyali, Melike (Science Direct, 2021)
    Nucleosomes form heterogeneous groups in vivo, named clutches. Clutches are smaller and less dense in mouse embryonic stem cells (ESCs) compared to neural progenitor cells (NPCs). Using coarse-grained modeling of the ...
  • (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes
    Neguembor, Maria Victoria; Sebastián Pérez, Rubén; Aulicino, Francesco, 1987-; Gómez García, Pablo; Cosma, Maria Pia, 1970-; Lakadamyali, Melike (Oxford University Press, 2018)
    CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization ...
  • SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state
    Sebastián Pérez, Rubén; Nakagawa, Shoma; Tu, Xiaochuan; Aranda Aragón, Sergio; Pesaresi, Martina; Gómez García, Pablo; Alcoverro-Bertran, Marc; Gómez Vázquez, José Luis; Carnevali, Davide; Borràs, Eva; Sabidó Aguadé, Eduard, 1981-; Martin, Laura; Nissim Rafinia, Malka; Meshorer, Eran; Neguembor, Maria Victoria; Di Croce, Luciano; Cosma, Maria Pia (eLife, 2023)
    Chromocenters are established after the 2-cell (2C) stage during mouse embryonic development, but the factors that mediate chromocenter formation remain largely unknown. To identify regulators of 2C heterochromatin ...
  • Super-resolution microscopy reveals how histone tail acetylation affects DNA compaction within nucleosomes in vivo
    Otterstrom, Jason; Castells García, Àlvaro, 1991-; Vicario, Chiara; Gómez García, Pablo; Cosma, Maria Pia, 1970-; Lakadamyali, Melike (Oxford University Press, 2019)
    Chromatin organization is crucial for regulating gene expression. Previously, we showed that nucleosomes form groups, termed clutches. Clutch size correlated with the pluripotency grade of mouse embryonic stem cells and ...
  • Transcription-mediated supercoiling regulates genome folding and loop formation
    Neguembor, Maria Victoria; Martin, Laura; Castells García, Àlvaro, 1991-; Gómez García, Pablo; Vicario, Chiara; Carnevali, Davide; Abed, Jumana AlHaj; Granados, Alba; Sebastián Pérez, Rubén; Sottile, Francesco, 1988-; Solon, Jérôme; Wu, Chao-Ting; Lakadamyali, Melike; Cosma, Maria Pia, 1970- (Elsevier, 2021)
    The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin ...
  • Two-parameter single-molecule analysis for measurement of chromatin mobility
    Lerner, Jonathan; Gómez García, Pablo; McCarthy, Ryan L.; Liu, Zhe; Lakadamyali, Melike; Zaret, Kenneth S. (Elsevier, 2020)
    This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin ...

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