Browsing by Author "Castanho, Miguel A.R.B."

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  • Conjugation of a blood brain barrier peptide shuttle to an Fc domain for brain delivery of therapeutic biomolecules
    Cavaco, Marco; Frutos, Silvia; Oliete, Paula; Valle, Javier; Andreu Martínez, David; Castanho, Miguel A.R.B.; Vila-Perelló, Miquel; Neves, Vera (American Chemical Society (ACS), 2021)
    The frequency of brain disease has increased significantly in the past years. After diagnosis, therapeutic options are usually limited, which demands the development of innovative therapeutic strategies. The use of ...
  • Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
    Cavaco, Marco; Fraga, Patrícia; Valle, Javier; Andreu Martínez, David; Castanho, Miguel A.R.B.; Neves, Vera (MDPI, 2021)
    Breast cancer (BC) is the most commonly diagnosed cancer in women and one of the most common causes of cancer-related deaths. Despite intense research efforts, BC treatment still remains challenging. Improved drug development ...
  • Estimating peptide half‐life in serum from tunable, sequence‐related physicochemical properties
    Cavaco, Marco; Valle, Javier; Flores, Isabel; Andreu Martínez, David; Castanho, Miguel A.R.B. (Wiley, 2021)
    Proteolytic instability is a critical limitation for peptide-based products. Although significant efforts are devoted to stabilize sequences against proteases/peptidases in plasma/serum, such approaches tend to be rather ...
  • Intracellular nucleic acid delivery by the supercharged dengue virus capsid protein
    Andreu Martínez, David; Miguel Freire, João; Veiga, Anna; Conceiçao, Thaís M.; Kowalczyk, Wioleta; Mohana-Borges, Ronaldo; Santos, Nuno C.; Da Poian, Andrea T.; Castanho, Miguel A.R.B. (Public Library of Science (PLoS), 2014)
    Supercharged proteins are a recently identified class of proteins that have the ability to efficiently deliver functional macromolecules into mammalian cells. They were first developed as bioengineering products, but were ...
  • Monitoring antibacterial permeabilization in real time using time-resolved flow cytometry
    Miguel Freire, João; Gaspar, Diana; García de la Torre, Beatriz; Veiga, Ana Salomé; Andreu Martínez, David; Castanho, Miguel A.R.B. (Elsevier, 2015)
    Despite the intensive study of antibiotic-induced bacterial permeabilization, its kinetics and molecular mechanism remain largely elusive. A new methodology that extends the concept of the live-dead assay in flow cytometry ...
  • New Potent Membrane-Targeting Antibacterial Peptides from Viral Capsid Proteins
    Dias, Susana A.; Miguel Freire, João; Pérez-Peinado, Clara; Domingues, Marco M.; Gaspar, Diana; Vale, Nuno; Gomes, Paula; Andreu Martínez, David; Henriques, Sónia T.; Castanho, Miguel A.R.B.; Veiga, Ana Salomé (Frontiers, 2017)
    The increasing prevalence of multidrug-resistant bacteria urges the development of new antibacterial agents. With a broad spectrum activity, antimicrobial peptides have been considered potential antibacterial drug leads. ...
  • Orally active peptide vector allows using cannabis to fight pain while avoiding side effects
    Gallo, María, 1989-; Moreno, Estefanía; Defaus, Sira; Ortega Alvaro, Antonio; Gonzalez, Angel; Robledo, Patricia, 1958-; Cavaco, Marco; Neves, Vera; Castanho, Miguel A.R.B.; Casadó, Vicent; Pardo Carrasco, Leonardo; Maldonado, Rafael, 1961-; Andreu Martínez, David (American Chemical Society (ACS), 2021)
    The activation of cannabinoid CB1 receptors (CB1R) by Δ9-tetrahydrocannabinol (THC), the main component of Cannabis sativa, induces analgesia. CB1R activation, however, also causes cognitive impairment via the serotonin ...
  • Penetrating the blood-brain barrier with new peptide-porphyrin conjugates having anti-HIV activity
    Mendonça, Diogo A.; Bakker, Mariët; Cruz-Oliveira, Christine; Neves, Vera; Jiménez, Maria Angeles; Defaus, Sira; Cavaco, Marco; Salomé Veiga, Ana; Cadima-Couto, Iris; Castanho, Miguel A.R.B.; Andreu Martínez, David; Todorovski, Toni (American Chemical Society (ACS), 2021)
    Passing through the blood-brain barrier (BBB) to treat neurological conditions is one of the main hurdles in modern medicine. Many drugs with promising in vitro profiles become ineffective in vivo due to BBB restrictive ...
  • Targeting Zika virus with new brain- and placenta-crossing peptide-porphyrin conjugates
    Todorovski, Toni; Mendonça, Diogo A.; Fernandes-Siqueira, Lorena O.; Cruz-Oliveira, Christine; Valle, Javier; Cavaco, Marco; Limas, Fernanda I. V.; Neves, Vera; Cadima-Couto, Iris; Defaus, Sira; Salomé Veiga, Ana; Da Poian, Andrea T.; Castanho, Miguel A.R.B.; Andreu Martínez, David (MDPI, 2022)
    Viral disease outbreaks affect hundreds of millions of people worldwide and remain a serious threat to global health. The current SARS-CoV-2 pandemic and other recent geographically- confined viral outbreaks (severe acute ...
  • The antimetastatic breast cancer activity of the viral protein-derived peptide vCPP2319 as revealed by cellular biomechanics
    Oliveira, Filipa D.; Cavaco, Marco; Figueira, Tiago N.; Valle, Javier; Neves, Vera; Andreu Martínez, David; Gaspar, Diana; Castanho, Miguel A.R.B. (Wiley, 2022)
    The incidence of metastatic breast cancer (MBC) is increasing and the therapeutic arsenal available to fight it is insufficient. Brain metastases, in particular, represent a major challenge for chemotherapy as the impermeable ...
  • The mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms
    Pinto, Sandra N.; Dias, Susana A.; Cruz, Ana F.; Mil-Homens, Dalila; Fernandes, Fabio; Valle, Javier; Andreu Martínez, David; Prieto, Manuel; Castanho, Miguel A.R.B.; Coutinho, Ana; Salomé Veiga, Ana (Oxford University Press, 2019)
    Objectives: To investigate the mechanism of action at the molecular level of pepR, a multifunctional peptide derived from the Dengue virus capsid protein, against Staphylococcus aureus biofilms. Methods: Biofilm mass, ...
  • To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
    Cavaco, Marco; Pérez-Peinado, Clara, 1991-; Valle, Javier; Silva, Rúben D.M.; Correia, João D.G.; Andreu Martínez, David; Castanho, Miguel A.R.B.; Neves, Vera (Frontiers, 2020)
    The characterization of biologically active peptides relies heavily on the study of their efficacy, toxicity, mechanism of action, cellular uptake, or intracellular location, using both in vitro and in vivo studies. These ...