Browsing by Author "Carolis, Carlo"

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  • A multiplex antigen microarray for simultaneous IgG and IgM detection against SARS-CoV-2 reveals higher seroprevalence than reported
    Ruano Gallego, David; García-Villadangos, Miriam; Moreno-Paz, Mercedes; Gómez-Elvira, Javier; Postigo, Marina; Simón-Sacristán, María; Reyburn, Hugh T.; Carolis, Carlo; Rodrigo, Natalia; Codeseira, Yaiza B.; Rueda, Paloma; Zúñiga, Sonia; Enjuanes, Luis; Parro, Victor (Wiley Open Access, 2021)
    The surge of SARS-CoV-2 has challenged health systems worldwide and efficient tests to detect viral particles, as well as antibodies generated against them, are needed. Specificity, sensitivity, promptness or scalability ...
  • Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain
    Dobaño, Carlota; Rodrigo Melero, Natalia; Carolis, Carlo; Jordan, Iolanda (BioMed Central, 2021)
    Background: Surveillance tools to estimate viral transmission dynamics in young populations are essential to guide recommendations for school opening and management during viral epidemics. Ideally, sensitive techniques are ...
  • Citizen science charts two major "stomatotypes" in the oral microbiome of adolescents and reveals links with habits and drinking water composition
    Willis, Jesse R.; González-Torres, Pedro; Pittis, Alexandros, 1982-; Bejarano, Luis A.; Cozzuto, Luca; Andreu Somavilla, Núria; Alloza-Trabado, Miriam; Valentín, Antònia; Ksiezopolska, Ewa; Company, Carlos; Onywera, Harris; Montfort, Magda; Hermoso Pulido, Antonio; Iraola Guzman, Susana; Saus Martínez, Ester; Labeeuw, Annick; Carolis, Carlo; Hecht, Jochen; Ponomarenko, Julia; Gabaldón Estevan, Juan Antonio, 1973- (BioMed Central, 2018)
    Background: The oral cavity comprises a rich and diverse microbiome, which plays important roles in health and disease. Previous studies have mostly focused on adult populations or in very young children, whereas the ...
  • Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species
    Willis, Jesse R.; Saus Martínez, Ester; Iraola Guzman, Susana; Cabello-Yeves, Elena; Ksiezopolska, Ewa; Cozzuto, Luca; Bejarano, Luis A.; Andreu Somavilla, Núria; Alloza-Trabado, Miriam; Blanco, Andrea; Puig-Sola, Anna; Broglio, Elisabetta; Carolis, Carlo; Ponomarenko, Julia; Hecht, Jochen; Gabaldón Estevan, Juan Antonio, 1973- (Taylor & Francis, 2021)
    Introduction: Cystic fibrosis (CF) is an autosomal genetic disease, associated with the production of excessively thick mucosa and with life-threatening chronic lung infections. The microbiota of the oral cavity can act ...
  • Citizen-science reveals changes in the oral microbiome in Spain through age and lifestyle factors
    Willis, Jesse R.; Saus Martínez, Ester; Iraola-Guzmán, Susana; Księżopolska, Ewa, 1987-; Cozzuto, Luca; Bejanaro, Luis A.; Andreu Somavilla, Núria; Alloza-Trabado, Miriam; Blanco, Andrea; Puig-Sola, Anna; Broglio, Elisabetta; Carolis, Carlo; Ponomarenko, Julia; Hecht, Jochen; Gabaldón Estevan, Juan Antonio, 1973- (Nature Research, 2022)
    The relevance of the human oral microbiome to our understanding of human health has grown in recent years as microbiome studies continue to develop. Given the links of the oral cavity with the digestive, respiratory and ...
  • Determinants of early antibody responses to COVID-19 mRNA vaccines in a cohort of exposed and naïve healthcare workers
    Moncunill, Gemma; Aguilar, Ruth; Ribes, Marta; Ortega, Natalia; Rubio, Rocío; Molina, María José; Vidal, Marta; Barrios, Diana; Mitchell, Robert A.; Jiménez, Alfons; Castellana, Cristina; Rodó, Pau; Méndez, Susana; Llupià, Anna; Puyol, Laura; Rodrigo Melero, Natalia; Carolis, Carlo; Mayor, Alfredo; Izquierdo, Luis; Trilla, Antoni; Vilella, Anna; García-Basteiro, Alberto L.; Dobaño, Carlota (Elsevier, 2022)
    Background: Two doses of mRNA vaccination have shown >94% efficacy at preventing COVID-19 mostly in naïve adults, but it is not clear if the second dose is needed to maximize effectiveness in those previously exposed to ...
  • Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants
    Sao Emani, Carine; Williams, Monique J.; Van Helden, Paul D.; Taylor, Malcolm J.C.; Carolis, Carlo; Wiid, I.J.; Baker, Bienyameen (Nature Research, 2018)
    Mycothiol (MSH) and ergothioneine (ERG) are thiols able to compensate for each other to protect mycobacteria against oxidative stress. Gamma-glutamylcysteine (GGC), another thiol and an intermediate in ERG biosynthesis has ...
  • Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes
    Vicencio, Jeremy, 1990-; Sánchez-Bolaños, Carlos; Moreno-Sánchez, Ismael; Brena, David; Vejnar, Charles E.; Kukhtar, Dmytro; Ruiz-López, Miguel; Cots-Ponjoan, Mariona; Rubio, Alejandro; Rodrigo Melero, Natalia; Crespo-Cuadrado, Jesús; Carolis, Carlo; Pérez-Pulido, Antonio J.; Giráldez, Antonio; Kleinstiver, Benjamin P.; Cerón Madrigal, Julián; Moreno Mateos, Miguel Ángel (Nature Research, 2022)
    The requirement for Cas nucleases to recognize a specific PAM is a major restriction for genome editing. SpCas9 variants SpG and SpRY, recognizing NGN and NRN PAMs, respectively, have contributed to increase the number of ...
  • In vivo force application reveals a fast tissue softening and external friction increase during early embryogenesis
    D'Angelo, Arturo; Dierkes, Kai; Carolis, Carlo; Salbreux, Guillaume; Solon, Jérôme (Elsevier, 2019)
    During development, cell-generated forces induce tissue-scale deformations to shape the organism [1,2]. The pattern and extent of these deformations depend not solely on the temporal and spatial profile of the generated ...
  • In-cell identification and measurement of RNA-protein interactions
    Graindorge, Antoine; Pinheiro, Inês; Nawrocka, Anna; Mallory, Allison C.; Tsvetkov, Peter; Gil, Noa; Carolis, Carlo; Buchholz, Frank; Ulitsky, Igor; Heard, Edith; Taipale, Mikko; Shkumatava, Alena (Nature Research, 2019)
    Regulatory RNAs exert their cellular functions through RNA-binding proteins (RBPs). Identifying RNA-protein interactions is therefore key for a molecular understanding of regulatory RNAs. To date, RNA-bound proteins have ...
  • Maternal and neonatal immune response to SARS-CoV-2, IgG transplacental transfer and cytokine profile
    Rubio, Rocío; Aguilar, Ruth; Bustamante Pineda, Mariona; Muñoz, Erica; Vázquez-Santiago, Miquel; Santano, Rebeca; Vidal, Marta; Rodrigo Melero, Natalia; Parras, Daniel; Serra, Pau; Santamaria, Pere; Carolis, Carlo; Izquierdo, Luis; Gómez-Roig, María Dolores; Dobaño, Carlota; Moncunill, Gemma; Mazarico, Edurne (Frontiers, 2022)
    SARS-CoV-2 infected pregnant women are at increased risk of severe COVID-19 than non-pregnant women and have a higher risk of adverse pregnancy outcomes like intrauterine/fetal distress and preterm birth. However, little ...
  • Multiplex antibody analysis of IgM, IgA and IgG to SARS-CoV-2 in saliva and serum from infected children and their close contacts
    Dobaño, Carlota; Alonso, Selena; Vidal, Marta; Jiménez, Alfons; Rubio, Rocío; Santano, Rebeca; Barrios, Diana; Puyol, Laura; Baró, Bàrbara; Millat-Martínez, Pere; Ajanovic, Sara; Balanza, Núria; Arias, Sara; Rodrigo Melero, Natalia; Carolis, Carlo; Bassat, Quique; Izquierdo, Luis; Aguilar, Ruth; Moncunill, Gemma (Frontiers, 2022)
    COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the ...
  • Oral microbiome in down syndrome and its implications on oral health
    Willis, Jesse R.; Iraola Guzman, Susana; Saus Martínez, Ester; Ksiezopolska, Ewa; Cozzuto, Luca; Bejarano, Luis A.; Andreu Somavilla, Núria; Alloza-Trabado, Miriam; Puig, Anna; Blanco, Andrea; Broglio, Elisabetta; Carolis, Carlo; Hecht, Jochen; Ponomarenko, Julia; Gabaldón Estevan, Juan Antonio, 1973- (Taylor & Francis Open Access, 2020)
    Introduction: The oral cavity harbors an abundant and diverse microbial community (i.e. the microbiome), whose composition and roles in health and disease have been the focus of intense research. Down syndrome (DS) is ...
  • Persistence and baseline determinants of seropositivity and reinfection rates in health care workers up to 12.5 months after COVID-19
    Dobaño, Carlota; Ramírez-Morros, Anna; Alonso, Selena; Vidal Alaball, Josep; Ruiz-Olalla, Gemma; Vidal, Marta; Rubio, Rocío; Cascant, Emma; Parras, Daniel; Rodrigo Melero, Natalia; Serra, Pau; Carolis, Carlo; Santamaria, Pere; Forcada, Anna; Mendioroz Peña, Jacobo; Aguilar, Ruth; Moncunill, Gemma; Ruiz-Comellas, Anna (BioMed Central, 2021)
    We assessed the duration and baseline determinants of antibody responses to SARS-CoV-2 spike antigens and the occurrence of reinfections in a prospective cohort of 173 Spanish primary health care worker patients followed ...
  • RBD-Based ELISA and luminex predict Anti-SARS-CoV-2 surrogate-neutralizing activity in two longitudinal cohorts of German and Spanish health care workers
    Aguilar, Ruth; Vidal, Marta; Rubio, Rocío; Jiménez, Alfons; Carolis, Carlo; Mayor, Alfredo; Izquierdo, Luis; Garcia Basteiro, Alberto L.; Moncunill, Gemma; Dobaño, Carlota; Gerhard, Markus (American Society for Microbiology, 2023)
    The ability of antibodies to neutralize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important correlate of protection. For routine evaluation of protection, however, a simple and cost-efficient ...
  • rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions
    Yang, Jae-Seong; Garriga-Canut, Mireia; Link, Nele; Carolis, Carlo; Broadbent, Katrina; Beltran-Sastre, Violeta; Serrano Pubull, Luis, 1982-; Maurer, Sebastian (Nature Research, 2018)
    Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed ...
  • Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study.
    López-Méndez, Blanca; Carolis, Carlo; Rodrigo, Natalia; Wu, Di (Springer, 2021)
    Microscale thermophoresis (MST), and the closely related Temperature Related Intensity Change (TRIC), are synonyms for a recently developed measurement technique in the field of biophysics to quantify biomolecular interactions, ...
  • SARS-CoV-2 infection, vaccination, and antibody response trajectories in adults: a cohort study in Catalonia
    Karachaliou, Marianna; Moncunill, Gemma; Espinosa Díaz, Ana; Castaño Vinyals, Gemma; Rubio, Rocío; Vidal, Marta; Jiménez, Alfons; Prados, Esther; Bañuls, Marc; Melero, Natalia Rodrigo; Izquierdo, Luis; Carolis, Carlo; Goldberg, Ximena; Aguilar, Ruth; García Aymerich, Judith; Kogevinas, Manolis; Dobaño, Carlota (BioMed Central, 2022)
    Background: Heterogeneity of the population in relation to infection, COVID-19 vaccination, and host characteristics is likely reflected in the underlying SARS-CoV-2 antibody responses. Methods: We measured IgM, IgA, and ...
  • SARS-CoV-2 sculpts the immune system to induce sustained virus-specific naïve-like and memory B-cell responses
    Campos Mata, Leire de, 1991-; Tejedor Vaquero, Sonia, 1988-; Tachó-Piñot, Roser; Piñero González, Janet, 1977-; Grasset, Emilie K.; Arrieta Aldea, Itziar; Rodrigo Melero, Natalia; Carolis, Carlo; Horcajada Gallego, Juan Pablo; Cerutti, Andrea, 1965-; Villar García, Judit; Magri, Giuliana, 1978- (Wiley Open Access, 2021)
    Objectives: SARS-CoV-2 infection induces virus-reactive memory B cells expressing unmutated antibodies, which hints at their emergence from naïve B cells. Yet, the dynamics of virus-specific naïve B cells and their impact ...
  • SARS-CoV-2 seroprevalence study in pediatric patients and health care workers using multiplex antibody immunoassays
    Prados de la Torre, Esther; Obando, Ignacio; Vidal, Marta; Felipe, Beatriz de; Aguilar, Ruth; Izquierdo, Luis; Carolis, Carlo; Olbrich, Peter; Capilla-Miranda, Ana; Serra, Pau; Santamaria, Pere; Blanco-Lobo, Pilar; Moncunill, Gemma; Rodríguez-Ortega, Manuel J.; Dobaño, Carlota (MDPI, 2022)
    SARS-CoV-2 infection has become a global health problem specially exacerbated with the continuous appearance of new variants. Healthcare workers (HCW) have been one of the most affected sectors. Children have also been ...