Activity of the δ-Opioid Receptor Is Partially Reduced, Whereas Activity of the κ-Receptor Is Maintained in Mice Lacking the μ-Receptor

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Matthes HWD, Smadja C, Valverde O, Vonesch JL, Foutz AS, Boudinot E, Denavit-Saubié M, Severini C, Negri L, Roques BP, Maldonado R, Kieffer BL. Activity of the δ-Opioid Receptor Is Partially Reduced, Whereas Activity of the κ-Receptor Is Maintained in Mice Lacking the μ-Receptor. J Neurosci. 1998; 18(18): 7285-95
http://hdl.handle.net/10230/16674
To cite or link this document: http://hdl.handle.net/10230/16674
dc.contributor.author Matthes, Hans W. D.
dc.contributor.author Smadja, C.
dc.contributor.author Valverde, Olga
dc.contributor.author Vonesch, J. L.
dc.contributor.author Foutz, A. S.
dc.contributor.author Boudinot, E.
dc.contributor.author Denavit-Saubié, M.
dc.contributor.author Severini, Cintia
dc.contributor.author Negri, Lucia
dc.contributor.author Roques, B. P.
dc.contributor.author Maldonado, Rafael, 1961-
dc.contributor.author Kieffer, Brigitte L.
dc.date.accessioned 2012-07-05T06:54:26Z
dc.date.available 2012-07-05T06:54:26Z
dc.date.issued 1998
dc.identifier.citation Matthes HWD, Smadja C, Valverde O, Vonesch JL, Foutz AS, Boudinot E, Denavit-Saubié M, Severini C, Negri L, Roques BP, Maldonado R, Kieffer BL. Activity of the δ-Opioid Receptor Is Partially Reduced, Whereas Activity of the κ-Receptor Is Maintained in Mice Lacking the μ-Receptor. J Neurosci. 1998; 18(18): 7285-95
dc.identifier.issn 0270-6474
dc.identifier.uri http://hdl.handle.net/10230/16674
dc.description.abstract Previous pharmacological studies have indicated the possible existence of functional interactions between μ-, δ- and κ-opioid receptors in the CNS. We have investigated this issue using a genetic approach. Here we describe in vitro and in vivo functional activity of δ- and κ-opioid receptors in mice lacking the μ-opioid receptor (MOR). Measurements of agonist-induced [35S]GTPγS binding and adenylyl cyclase inhibition showed that functional coupling of δ- and κ-receptors to G-proteins is preserved in the brain of mutant mice. In the mouse vas deferens bioassay, deltorphin II and cyclic[d-penicillamine2,d-penicillamine5] enkephalin exhibited similar potency to inhibit smooth muscle contraction in both wild-type and MOR −/− mice. δ-Analgesia induced by deltorphin II was slightly diminished in mutant mice, when the tail flick test was used. Deltorphin II strongly reduced the respiratory frequency in wild-type mice but not in MOR −/− mice. Analgesic and respiratory responses produced by the selective κ-agonist U-50,488H were unchanged in MOR-deficient mice. In conclusion, the preservation of δ- and κ-receptor signaling properties in mice lacking μ-receptors provides no evidence for opioid receptor cross-talk at the cellular level. Intact antinociceptive and respiratory responses to the κ-agonist further suggest that the κ-receptor mainly acts independently from the μ-receptor in vivo. Reduced δ-analgesia and the absence of δ-respiratory depression in MOR-deficient mice together indicate that functional interactions may take place between μ-receptors and central δ-receptors in specific neuronal pathways.
dc.language.iso eng
dc.publisher Society for Neuroscience
dc.relation.ispartof J Neurosci. 1998; 18(18): 7285-95
dc.rights (c) 1998, Society for Neuroscience. The published version is available at: http://www.jneurosci.org/content/18/18/7285
dc.subject.other Opiacis -- Receptors
dc.subject.other Drogoaddicció -- Aspectes moleculars
dc.title Activity of the δ-Opioid Receptor Is Partially Reduced, Whereas Activity of the κ-Receptor Is Maintained in Mice Lacking the μ-Receptor
dc.type info:eu-repo/semantics/article
dc.subject.keyword μ-opioid receptor knock-out
dc.subject.keyword μ-δ-κ-opioid receptor interactions
dc.subject.keyword G-protein coupling
dc.subject.keyword δ-κ analgesia
dc.subject.keyword Respiration
dc.subject.keyword Vas deferens
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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