Genome-wide screen of genes required for caffeine tolerance in fission yeast

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Calvo IA, Gabrielli N, Iglesias-Baena I, García-Santamarina S, Hoe K-L, Kim DU, Sansó M, Zuin A, Pérez P, Ayté J, Hidalgo E. Genome-Wide Screen of Genes Required for Caffeine Tolerance in Fission Yeast. PLoS ONE. 2009; 4(8): e6619. DOI:10.1371/journal.pone.0006619
http://hdl.handle.net/10230/16364
To cite or link this document: http://hdl.handle.net/10230/16364
dc.contributor.author Calvo, Isabel A.
dc.contributor.author Gabrielli, Natalia
dc.contributor.author Iglesias Baena, Iván
dc.contributor.author García Santamarina, Sarela
dc.contributor.author Hoe, Kwang-Lae
dc.contributor.author Kim, Dong Uk
dc.contributor.author Sansó Martínez, Miriam
dc.contributor.author Zuin, Alice
dc.contributor.author Pérez, Pilar
dc.contributor.author Ayté del Olmo, José
dc.contributor.author Hidalgo Hernando, Elena
dc.date.accessioned 2012-04-16T16:05:36Z
dc.date.available 2012-04-16T16:05:36Z
dc.date.issued 2009
dc.identifier.citation Calvo IA, Gabrielli N, Iglesias-Baena I, García-Santamarina S, Hoe K-L, Kim DU, Sansó M, Zuin A, Pérez P, Ayté J, Hidalgo E. Genome-Wide Screen of Genes Required for Caffeine Tolerance in Fission Yeast. PLoS ONE. 2009; 4(8): e6619. DOI:10.1371/journal.pone.0006619
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10230/16364
dc.description.abstract Background: An excess of caffeine is cytotoxic to all eukaryotic cell types. We aim to study how cells become tolerant to a toxic dose of this drug, and the relationship between caffeine and oxidative stress pathways. Methodology/Principal Findings: We searched for Schizosaccharomyces pombe mutants with inhibited growth on caffeinecontaining plates. We screened a collection of 2,700 haploid mutant cells, of which 98 were sensitive to caffeine. The genes mutated in these sensitive clones were involved in a number of cellular roles including the H2O2-induced Pap1 and Sty1 stress pathways, the integrity and calcineurin pathways, cell morphology and chromatin remodeling. We have investigated the role of the oxidative stress pathways in sensing and promoting survival to caffeine. The Pap1 and the Sty1 pathways are both required for normal tolerance to caffeine, but only the Sty1 pathway is activated by the drug. Cells lacking Pap1 are sensitive to caffeine due to the decreased expression of the efflux pump Hba2. Indeed, ?hba2 cells are sensitive to caffeine, and constitutive activation of the Pap1 pathway enhances resistance to caffeine in an Hba2-dependent manner. Conclusions/Significance: With our caffeine-sensitive, genome-wide screen of an S. pombe deletion collection, we have demonstrated the importance of some oxidative stress pathway components on wild-type tolerance to the drug.
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS ONE. 2009; 4(8): e6619
dc.rights © 2009 Calvo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/2.5/
dc.subject.other Cafeïna
dc.subject.other Cèl·lules eucariotes -- Aspectes genètics
dc.title Genome-wide screen of genes required for caffeine tolerance in fission yeast
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0006619
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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