González F, Barragan M, Tiscornia G, Montserrat N, Vassena R, Batlle L et al. Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector. Proc Natl Acad Sci U S A. 2009; 106(22): 8918–8922. DOI 10.1073/pnas.0901471106
http://hdl.handle.net/10230/12427
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Title:
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Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector |
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Author:
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Rodríguez Pizà, Ignasi; Barragan, Montserrat; González, Federico; Izpisúa Belmonte, J. C.; Batlle Morera, Laura; Vassena, Rita; Montserrat, Nuria; Tiscornia, Gustavo
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Abstract:
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Induced pluripotent stem (iPS) cells have generated keen interest
due to their potential use in regenerative medicine. They have
been obtained from various cell types of both mice and humans by
exogenous delivery of different combinations of Oct4, Sox2, Klf4,
c-Myc, Nanog, and Lin28. The delivery of these transcription factors
has mostly entailed the use of integrating viral vectors (retroviruses
or lentiviruses), carrying the risk of both insertional mutagenesis
and oncogenesis due to misexpression of these exogenous
factors. Therefore, obtaining iPS cells that do not carry integrated
transgene sequences is an important prerequisite for their eventual
therapeutic use. Here we report the generation of iPS cell lines
from mouse embryonic fibroblasts with no evidence of integration
of the reprogramming vector in their genome, achieved by nucleofection
of a polycistronic construct coexpressing Oct4, Sox2, Klf4,
and c-Myc
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Document type:
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Article
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Document version:
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Published version
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Date:
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2009 |
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Rights:
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(c) National Academy of Sciences |
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