Analysis of mouse kreisler mutants reveals new roles of hindbrain-derived signals in the establishment of the otic neurogenic domain

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Vázquez Echeverría, Citlali. Analysis of mouse kreisler mutants reveals new roles of hindbrain-derived signals in the establishment of the otic neurogenic domain. 2008
http://hdl.handle.net/10230/12191
dc.contributor.author Vázquez Echeverría, Citlali
dc.contributor.other Pujades Corbi, Cristina
dc.contributor.other Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
dc.date.accessioned 2013-07-12T01:51:08Z
dc.date.available 2013-07-12T01:51:08Z
dc.date.issued 2008-12-18
dc.identifier.uri http://hdl.handle.net/10230/12191
dc.description.abstract The inner ear, the sensory organ responsible for hearing and balance, contains specialized sensory and non-sensory epithelia arranged in a highly complex threedimensional structure. To achieve this complexity, a tight coordination between morphogenesis and cell fate specification is essential during otic development. Tisúes surrounding the otic primordium, and more particularly the adjacent segmented hindbrain, have been implicated in specifying structures along the anteroposterior and dorsoventral axes of the inner ear. In this work we have first characterized the generation and axial specification of the otic neurogenic domain, and second, we have investigated the effects of the mutation of kreisler/MafB -a gene transiently expressed in the rhombomeres 5 and 6 of the developing hindbrain- in early otic patterning and cell specification. We show that kr/kr embryos display an expansion of the otic neurogenic domain, due to defects in otic patterning. Although many reports have pointed to the role of FGF3 in otic regionalization, we provide evidence that FGF3 is not sufficient to govern this process. Neither Krox20 nor Fgf3 null mutant embryos, in which Fgf3 is either downregulated or absent in r5 and r6, present ectopic otic neuroblasts in the otic primordium. However, Fgf3-/-Fgf10-/- double mutants show a phenotype very similar to kr/kr embryos: they present ectopic neuroblasts along the AP and DV otic axes. Finally, and remarkably, partial rescue of the kr/kr phenotype is obtained when Fgf3 or Fgf10 are ectopically expressed in the hindbrain of kr/kr embryos. These results highlight a compensatory mechanism between FGFs, and the importance of hindbrain-derived signals in instructing otic patterning and the establishment of the neurogenic domain.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Universitat Pompeu Fabra
dc.rights ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.
dc.rights info:eu-repo/semantics/openAccess
dc.title Analysis of mouse kreisler mutants reveals new roles of hindbrain-derived signals in the establishment of the otic neurogenic domain
dc.date.modified 2013-07-10T11:29:09Z
dc.subject.keyword hindbrain
dc.subject.keyword inner ear
dc.subject.keyword patterning
dc.subject.keyword labyrinth (ear) - physiology
dc.subject.keyword kreisler/MafB
dc.subject.keyword rhombencephalon
dc.subject.keyword mice as laboratory animals - embriology
dc.subject.keyword developmental neurobiology
dc.subject.keyword laberint (orella) - fisiologia
dc.subject.keyword romboencèfal
dc.subject.keyword ratolins (animals de laboratori) - embriologia
dc.subject.keyword neurobiologia del desenvolupament
dc.subject.keyword FGF
dc.subject.keyword Krox20
dc.subject.keyword neurogenesis
dc.subject.keyword 59
dc.subject.keyword 616.8

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http://hdl.handle.net/10803/7157

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